Trigeminal nociceptors express prostaglandin receptors

Amol M Patwardhan, J. Vela, J. Farugiq, K. Vela, K. M. Hargreaves

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Orofacial inflammation is associated with prostaglandin release and the sensitization of nociceptive receptors such as the transient receptor potential subtype V1 (TRPV1). We hypothesized that certain PGE 2 receptor subtypes (EP1-EP4) are co-expressed with TRPV1 in trigeminal nociceptors and sensitize responses to a TRPV1 agonist, capsaicin. Accordingly, combined in situ hybridization was performed with immunohisto-chemistry on rat trigeminal ganglia. We next evaluated the effects of specific EP2 and EP3 agonists (butaprost and sulprostone) in cultured trigeminal ganglia neurons. The results showed that EP2 and EP3 are expressed in trigeminal neurons (58% and 53% of total neurons, respectively) and are co-expressed in TRPV1-positive neurons (64% and 67 % of TRPV 1-positive neurons, respectively). Moreover, most of the cells expressing EP2 or EP3 mRNA were of small to medium diameter (< 30 μm). The application of butaprost and sulprostone triggered neuropeptide exocytosis, and butaprost sensitized capsaicin responses. Analysis of these data, collectively, supports the hypothesis that prostaglandins regulate trigeminal TRPV 1 nociceptors via activation of the EP2 and EP3 receptors.

Original languageEnglish (US)
Pages (from-to)262-266
Number of pages5
JournalJournal of Dental Research
Volume87
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

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Prostaglandin Receptors
Nociceptors
Neurons
Trigeminal Ganglion
Capsaicin
Prostaglandins
Receptors, Prostaglandin E, EP1 Subtype
Exocytosis
Neuropeptides
In Situ Hybridization
Inflammation
Messenger RNA
butaprost

Keywords

  • CGRP
  • PGE
  • Trigeminal
  • TRPV

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Trigeminal nociceptors express prostaglandin receptors. / Patwardhan, Amol M; Vela, J.; Farugiq, J.; Vela, K.; Hargreaves, K. M.

In: Journal of Dental Research, Vol. 87, No. 3, 03.2008, p. 262-266.

Research output: Contribution to journalArticle

Patwardhan, Amol M ; Vela, J. ; Farugiq, J. ; Vela, K. ; Hargreaves, K. M. / Trigeminal nociceptors express prostaglandin receptors. In: Journal of Dental Research. 2008 ; Vol. 87, No. 3. pp. 262-266.
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