Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men

Results from single lumbar puncture sampling technique

Francisco Moreno, Cameron McGavin, T. Phillip Malan, Alan J. Gelenberg, George R. Heninger, Aleksander A. Mathé, Pedro L. Delgado

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Plasma tryptophan (Trp) depletion is a commonly used tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders. This study measured the cerebrospinal fluid (CSF) monoamine metabolite response to Trp depletion and control testing in five healthy subjects utilizing a single lumbar puncture. Testing was done in a placebo-controlled, double-blind, randomized, cross-over design. Plasma-free and total Trp levels and behavioural ratings were obtained prior to and 5 h after ingestion of each amino-acid drink. CSF was obtained by performing a standard lumbar puncture 7 h after ingestion of the drink. Compared to control testing, Trp depletion caused a significant decrease of CSF 5-hydroxyindoleacetic acid (5-HIAA) (p = 0.03), but not of homovanillic acid or 3-methoxy-4-hydroxy-phenylglycol. Behavioural ratings were minimally affected in all subjects. This confirms that plasma Trp depletion reduces central nervous system measures of 5-HT function and suggests that the single lumbar puncture technique may be sufficient to detect the extent of CSF 5-HIAA changes during Trp depletion studies.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalInternational Journal of Neuropsychopharmacology
Volume3
Issue number4
DOIs
StatePublished - 2000

Fingerprint

Spinal Puncture
Tryptophan
Cerebrospinal Fluid
Serotonin
Hydroxyindoleacetic Acid
Eating
Homovanillic Acid
Cross-Over Studies
Psychiatry
Healthy Volunteers
Central Nervous System
Placebos
Amino Acids
Brain

Keywords

  • Cerebrospinal fluid (CSF)
  • Monoamine metabolites
  • Tryptophan depletion

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Neuropsychology and Physiological Psychology
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men : Results from single lumbar puncture sampling technique. / Moreno, Francisco; McGavin, Cameron; Phillip Malan, T.; Gelenberg, Alan J.; Heninger, George R.; Mathé, Aleksander A.; Delgado, Pedro L.

In: International Journal of Neuropsychopharmacology, Vol. 3, No. 4, 2000, p. 277-283.

Research output: Contribution to journalArticle

Moreno, Francisco ; McGavin, Cameron ; Phillip Malan, T. ; Gelenberg, Alan J. ; Heninger, George R. ; Mathé, Aleksander A. ; Delgado, Pedro L. / Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men : Results from single lumbar puncture sampling technique. In: International Journal of Neuropsychopharmacology. 2000 ; Vol. 3, No. 4. pp. 277-283.
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