Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: Results from single lumbar puncture sampling technique

Francisco Moreno, Cameron McGavin, T. Phillip Malan, Alan J. Gelenberg, George R. Heninger, Aleksander A. Mathé, Pedro L. Delgado

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Plasma tryptophan (Trp) depletion is a commonly used tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders. This study measured the cerebrospinal fluid (CSF) monoamine metabolite response to Trp depletion and control testing in five healthy subjects utilizing a single lumbar puncture. Testing was done in a placebo-controlled, double-blind, randomized, cross-over design. Plasma-free and total Trp levels and behavioural ratings were obtained prior to and 5 h after ingestion of each amino-acid drink. CSF was obtained by performing a standard lumbar puncture 7 h after ingestion of the drink. Compared to control testing, Trp depletion caused a significant decrease of CSF 5-hydroxyindoleacetic acid (5-HIAA) (p = 0.03), but not of homovanillic acid or 3-methoxy-4-hydroxy-phenylglycol. Behavioural ratings were minimally affected in all subjects. This confirms that plasma Trp depletion reduces central nervous system measures of 5-HT function and suggests that the single lumbar puncture technique may be sufficient to detect the extent of CSF 5-HIAA changes during Trp depletion studies.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalInternational Journal of Neuropsychopharmacology
Volume3
Issue number4
DOIs
StatePublished - 2000

Fingerprint

Spinal Puncture
Tryptophan
Cerebrospinal Fluid
Serotonin
Hydroxyindoleacetic Acid
Eating
Homovanillic Acid
Cross-Over Studies
Psychiatry
Healthy Volunteers
Central Nervous System
Placebos
Amino Acids
Brain

Keywords

  • Cerebrospinal fluid (CSF)
  • Monoamine metabolites
  • Tryptophan depletion

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Neuropsychology and Physiological Psychology
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men : Results from single lumbar puncture sampling technique. / Moreno, Francisco; McGavin, Cameron; Phillip Malan, T.; Gelenberg, Alan J.; Heninger, George R.; Mathé, Aleksander A.; Delgado, Pedro L.

In: International Journal of Neuropsychopharmacology, Vol. 3, No. 4, 2000, p. 277-283.

Research output: Contribution to journalArticle

Moreno, Francisco ; McGavin, Cameron ; Phillip Malan, T. ; Gelenberg, Alan J. ; Heninger, George R. ; Mathé, Aleksander A. ; Delgado, Pedro L. / Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men : Results from single lumbar puncture sampling technique. In: International Journal of Neuropsychopharmacology. 2000 ; Vol. 3, No. 4. pp. 277-283.
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