tudor, a posterior-group gene of Drosophila melanogaster, encodes a novel protein and an mRNA localized during mid-oogenesis

George S. Golumbeski, Anne Bardsley, Frans Tax, Robert E. Boswell

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The tudor (tud) locus of Drosophila melanogaster is required during oogenesis for the formation of primordial germ cells and for normal abdominal segmentation. The tud locus was cloned, and its product was identified by Northern analysis of wild-type and tud mutant RNAs. The locus encodes a single mRNA of ∼8.0 kb that is expressed throughout the life cycle, beginning in the early stages of germ-line development in the female. During oogenesis, tud mRNA appears to be present in the oocyte precursor within the germarial cysts, and in stages 1-3 it accumulates within the developing oocyte. The transcript is localized to the posterior half of the oocyte during oogenetic stages 4-7 but is not detectable within the ooplasm by egg deposition and throughout early embryogenesis. The tud protein has a predicted molecular mass of 285,000 daltons and has no distinctive sequence similarity to known proteins or protein structural motifs. Taken together, these results indicate that the tud product is a novel protein required during oogenesis for establishment of a functional center of morphogenetic activity in the posterior tip of the Drosophila embryo.

Original languageEnglish (US)
Pages (from-to)2060-2070
Number of pages11
JournalGenes and Development
Volume5
Issue number11
StatePublished - Nov 1991
Externally publishedYes

Fingerprint

Oogenesis
Drosophila melanogaster
Oocytes
Germ Cells
Messenger RNA
Genes
Amino Acid Motifs
Proteins
Life Cycle Stages
Drosophila
Embryonic Development
Ovum
Cysts
Embryonic Structures
RNA

Keywords

  • Drosophila melanogaster
  • Oogenesis
  • Pattern formation
  • Tudor

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

tudor, a posterior-group gene of Drosophila melanogaster, encodes a novel protein and an mRNA localized during mid-oogenesis. / Golumbeski, George S.; Bardsley, Anne; Tax, Frans; Boswell, Robert E.

In: Genes and Development, Vol. 5, No. 11, 11.1991, p. 2060-2070.

Research output: Contribution to journalArticle

Golumbeski, George S. ; Bardsley, Anne ; Tax, Frans ; Boswell, Robert E. / tudor, a posterior-group gene of Drosophila melanogaster, encodes a novel protein and an mRNA localized during mid-oogenesis. In: Genes and Development. 1991 ; Vol. 5, No. 11. pp. 2060-2070.
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