Tumor stroma as the main source of inhibin production in ovarian epithelial tumors

Wenxin - Zheng, Jean J. Lu, Feng Luo, John Hsieh, Chun Yeh Wang, Chunying Zhang, Lilly Chang, Michael M. Cho, Frank Z. Stanczyk

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

PROBLEM: Elevated serum inhibin levels have been found in ovarian cancer patients; however, the source of the elevated inhibin is uncertain. Previous studies of activin in human ovarian cancer suggest that activin may promote the growth of the tumor. The aims of this study were to examine the source of elevated inhibin from ovarian epithelial tumors (OETs) and to preliminarily investigate the role of the gonadotropin-inhibin/activin relationship in the development of OET. METHOD OF STUDY: The protein and mRNA expression of a and PA subunits of inhibin/activin were examined by immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR) in 120 OETs, including 30 benign cystadenomas, 30 borderline tumors, and 60 carcinomas. Stromal and epithelial cells were microdissected from 23 OETs to further examine the expression of α and βA subunits by RT-PCR. Dimeric inhibin A and activin A production were measured by using the two-site ELISA from three OET cell lines in culture under treatment of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). RESULTS: PA subunit was expressed in the epithelial component of 100% of the cystadenomas, in 80% of borderline tumors, and in 75% of the carcinomas, but not in tumor stroma. Inhibin a expression was not found in the epithelium of all OETs studied, but focal inhibin a immunoreactivity was seen in the tumor stroma (mainly luteinized stromal cells) in the majority of cases. Dimeric activin A was produced by all of the three OET cell lines with a 1.5-1.9-fold increment after FSH stimulation. However, activin A production was not augmented by LH treatment. No inhibin A was produced by the three OET cell lines with or without gonadotropin stimulation. CONCLUSIONS: The stroma of OET is the major source in the production of inhibin α (monomer). Dimeric inhibin A production may be the result of combined efforts of the tumor stroma (α subunit) and epithelium (βA subunit). Cellular, compartmental expression of inhibin and activin subunits may play a role in the development of OET, although the mechanism remains undefined. The unopposed activin A production stimulated by FSH in OET cell lines suggests that activin production may represent one of the cellular mechanisms of growth promotion by FSH.

Original languageEnglish (US)
Pages (from-to)104-113
Number of pages10
JournalAmerican Journal of Reproductive Immunology
Volume44
Issue number2
StatePublished - Aug 2000
Externally publishedYes

Fingerprint

Inhibins
Activins
Neoplasms
Follicle Stimulating Hormone
Tumor Cell Line
Epithelial Cells
Cystadenoma
Stromal Cells
Luteinizing Hormone
Gonadotropins
Ovarian Neoplasms
Reverse Transcription
Epithelium
Carcinoma
Polymerase Chain Reaction
Growth

Keywords

  • Activin
  • Gonadotropin
  • Inhibin
  • Ovarian cancer

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Zheng, W. ., Lu, J. J., Luo, F., Hsieh, J., Wang, C. Y., Zhang, C., ... Stanczyk, F. Z. (2000). Tumor stroma as the main source of inhibin production in ovarian epithelial tumors. American Journal of Reproductive Immunology, 44(2), 104-113.

Tumor stroma as the main source of inhibin production in ovarian epithelial tumors. / Zheng, Wenxin -; Lu, Jean J.; Luo, Feng; Hsieh, John; Wang, Chun Yeh; Zhang, Chunying; Chang, Lilly; Cho, Michael M.; Stanczyk, Frank Z.

In: American Journal of Reproductive Immunology, Vol. 44, No. 2, 08.2000, p. 104-113.

Research output: Contribution to journalArticle

Zheng, W, Lu, JJ, Luo, F, Hsieh, J, Wang, CY, Zhang, C, Chang, L, Cho, MM & Stanczyk, FZ 2000, 'Tumor stroma as the main source of inhibin production in ovarian epithelial tumors', American Journal of Reproductive Immunology, vol. 44, no. 2, pp. 104-113.
Zheng, Wenxin - ; Lu, Jean J. ; Luo, Feng ; Hsieh, John ; Wang, Chun Yeh ; Zhang, Chunying ; Chang, Lilly ; Cho, Michael M. ; Stanczyk, Frank Z. / Tumor stroma as the main source of inhibin production in ovarian epithelial tumors. In: American Journal of Reproductive Immunology. 2000 ; Vol. 44, No. 2. pp. 104-113.
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abstract = "PROBLEM: Elevated serum inhibin levels have been found in ovarian cancer patients; however, the source of the elevated inhibin is uncertain. Previous studies of activin in human ovarian cancer suggest that activin may promote the growth of the tumor. The aims of this study were to examine the source of elevated inhibin from ovarian epithelial tumors (OETs) and to preliminarily investigate the role of the gonadotropin-inhibin/activin relationship in the development of OET. METHOD OF STUDY: The protein and mRNA expression of a and PA subunits of inhibin/activin were examined by immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR) in 120 OETs, including 30 benign cystadenomas, 30 borderline tumors, and 60 carcinomas. Stromal and epithelial cells were microdissected from 23 OETs to further examine the expression of α and βA subunits by RT-PCR. Dimeric inhibin A and activin A production were measured by using the two-site ELISA from three OET cell lines in culture under treatment of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). RESULTS: PA subunit was expressed in the epithelial component of 100{\%} of the cystadenomas, in 80{\%} of borderline tumors, and in 75{\%} of the carcinomas, but not in tumor stroma. Inhibin a expression was not found in the epithelium of all OETs studied, but focal inhibin a immunoreactivity was seen in the tumor stroma (mainly luteinized stromal cells) in the majority of cases. Dimeric activin A was produced by all of the three OET cell lines with a 1.5-1.9-fold increment after FSH stimulation. However, activin A production was not augmented by LH treatment. No inhibin A was produced by the three OET cell lines with or without gonadotropin stimulation. CONCLUSIONS: The stroma of OET is the major source in the production of inhibin α (monomer). Dimeric inhibin A production may be the result of combined efforts of the tumor stroma (α subunit) and epithelium (βA subunit). Cellular, compartmental expression of inhibin and activin subunits may play a role in the development of OET, although the mechanism remains undefined. The unopposed activin A production stimulated by FSH in OET cell lines suggests that activin production may represent one of the cellular mechanisms of growth promotion by FSH.",
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AU - Zheng, Wenxin -

AU - Lu, Jean J.

AU - Luo, Feng

AU - Hsieh, John

AU - Wang, Chun Yeh

AU - Zhang, Chunying

AU - Chang, Lilly

AU - Cho, Michael M.

AU - Stanczyk, Frank Z.

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N2 - PROBLEM: Elevated serum inhibin levels have been found in ovarian cancer patients; however, the source of the elevated inhibin is uncertain. Previous studies of activin in human ovarian cancer suggest that activin may promote the growth of the tumor. The aims of this study were to examine the source of elevated inhibin from ovarian epithelial tumors (OETs) and to preliminarily investigate the role of the gonadotropin-inhibin/activin relationship in the development of OET. METHOD OF STUDY: The protein and mRNA expression of a and PA subunits of inhibin/activin were examined by immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR) in 120 OETs, including 30 benign cystadenomas, 30 borderline tumors, and 60 carcinomas. Stromal and epithelial cells were microdissected from 23 OETs to further examine the expression of α and βA subunits by RT-PCR. Dimeric inhibin A and activin A production were measured by using the two-site ELISA from three OET cell lines in culture under treatment of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). RESULTS: PA subunit was expressed in the epithelial component of 100% of the cystadenomas, in 80% of borderline tumors, and in 75% of the carcinomas, but not in tumor stroma. Inhibin a expression was not found in the epithelium of all OETs studied, but focal inhibin a immunoreactivity was seen in the tumor stroma (mainly luteinized stromal cells) in the majority of cases. Dimeric activin A was produced by all of the three OET cell lines with a 1.5-1.9-fold increment after FSH stimulation. However, activin A production was not augmented by LH treatment. No inhibin A was produced by the three OET cell lines with or without gonadotropin stimulation. CONCLUSIONS: The stroma of OET is the major source in the production of inhibin α (monomer). Dimeric inhibin A production may be the result of combined efforts of the tumor stroma (α subunit) and epithelium (βA subunit). Cellular, compartmental expression of inhibin and activin subunits may play a role in the development of OET, although the mechanism remains undefined. The unopposed activin A production stimulated by FSH in OET cell lines suggests that activin production may represent one of the cellular mechanisms of growth promotion by FSH.

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KW - Gonadotropin

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