Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6

Jeffrey M. Trent, Eric J. Stanbridge, Heyoung L. Mcbride, Eckart U. Meese, Graham Casey, Diana E. Araujo, Colette M. Witkowski, Raymond B Nagle

Research output: Contribution to journalArticle

303 Citations (Scopus)

Abstract

Chromosome banding analysis of human malignant melanoma has documented the nonrandom alteration of chromosome 6. To determine the relevance of chromosome 6 abnormalities in melanoma, a normal chromosome 6 was directly introduced into melanoma cell lines. The resulting (+6) microcell hybrids were significantly altered in their phenotypic properties in culture and lost their ability to form tumors in nude mice. The loss of the chromosome 6 from melanoma microcell hybrids resulted in the reversion to tumorigenicity of these cells in mice. The introduction of the selectable marker (psv2neo) alone into melanoma cell lines had no effect on tumorigenicity. These results support the idea that one or more genes on chromosome 6 may control the malignant expression of human melanoma.

Original languageEnglish (US)
Pages (from-to)568-571
Number of pages4
JournalScience
Volume247
Issue number4942
StatePublished - Feb 2 1990

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Chromosomes, Human, Pair 6
Human Chromosomes
Melanoma
Cell Line
Chromosome Banding
Nude Mice
Chromosome Aberrations
Genes
Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Trent, J. M., Stanbridge, E. J., Mcbride, H. L., Meese, E. U., Casey, G., Araujo, D. E., ... Nagle, R. B. (1990). Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6. Science, 247(4942), 568-571.

Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6. / Trent, Jeffrey M.; Stanbridge, Eric J.; Mcbride, Heyoung L.; Meese, Eckart U.; Casey, Graham; Araujo, Diana E.; Witkowski, Colette M.; Nagle, Raymond B.

In: Science, Vol. 247, No. 4942, 02.02.1990, p. 568-571.

Research output: Contribution to journalArticle

Trent, JM, Stanbridge, EJ, Mcbride, HL, Meese, EU, Casey, G, Araujo, DE, Witkowski, CM & Nagle, RB 1990, 'Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6', Science, vol. 247, no. 4942, pp. 568-571.
Trent JM, Stanbridge EJ, Mcbride HL, Meese EU, Casey G, Araujo DE et al. Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6. Science. 1990 Feb 2;247(4942):568-571.
Trent, Jeffrey M. ; Stanbridge, Eric J. ; Mcbride, Heyoung L. ; Meese, Eckart U. ; Casey, Graham ; Araujo, Diana E. ; Witkowski, Colette M. ; Nagle, Raymond B. / Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6. In: Science. 1990 ; Vol. 247, No. 4942. pp. 568-571.
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abstract = "Chromosome banding analysis of human malignant melanoma has documented the nonrandom alteration of chromosome 6. To determine the relevance of chromosome 6 abnormalities in melanoma, a normal chromosome 6 was directly introduced into melanoma cell lines. The resulting (+6) microcell hybrids were significantly altered in their phenotypic properties in culture and lost their ability to form tumors in nude mice. The loss of the chromosome 6 from melanoma microcell hybrids resulted in the reversion to tumorigenicity of these cells in mice. The introduction of the selectable marker (psv2neo) alone into melanoma cell lines had no effect on tumorigenicity. These results support the idea that one or more genes on chromosome 6 may control the malignant expression of human melanoma.",
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