Abstract
This work presents a novel technique using a reverse potential electrospinning mode for fabricating nanoparticle-embedded composites that can be tailored to represent various fiber diameters, surface morphologies, and functional groups necessary for improved cellular adhesion. Polycaprolactone (PCL) nanofibers were electrospun in both traditional positive (PP) and reverse potential (RP) electrical fields. The fibers were incorporated with 300 nm polystyrene (PS) fluorescent particles, which contained carboxyl, amine groups, and surfactants. In the unconventional RP, the charged colloidal particles and surfactants were shown to have an exaggerated effect on Taylor cone morphology and fiber diameter caused by the changes in charge density and surface tension of the bulk solution. The RP mode was shown to lead to a decrease in fiber diameter from 1200 ± 100 nm (diameter ± SE) for the nanofibers made with PCL alone to 440 ± 80 nm with the incorporation of colloidal particles, compared to the PP mode ranging from 530 ± 90 nm to 350 ± 50 nm, respectively. The nanoparticle-nanofiber composite substrates were cultured with human umbilical vein endothelial cells (HUVECs) and evaluated for cellular viability and adhesion for up to 5 days. Adhesion to the nanofibrous substrates was improved by 180 ± 10% with the addition of carboxylated particles and by 480 ± 60% with the functionalization of an RGD ligand compared to the PCL nanofibers. The novel approach of electrospinning in the RP mode with the addition of colloids in order to alter charge density and surface tension could be utilized towards many applications, one being implantable biomaterials and tissue engineered scaffolds as demonstrated in this work.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 830-838 |
| Number of pages | 9 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 145 |
| DOIs | |
| State | Published - Sep 1 2016 |
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Keywords
- Functionalized nanoparticles
- Reverse potential electrospinning
- RGD ligand
- Surface tension
- Surfactant
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Tuneable nanoparticle-nanofiber composite substrate for improved cellular adhesion. / Nicolini, Ariana M.; Toth, Tyler D.; Yoon, Jeong-Yeol.
In: Colloids and Surfaces B: Biointerfaces, Vol. 145, 01.09.2016, p. 830-838.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tuneable nanoparticle-nanofiber composite substrate for improved cellular adhesion
AU - Nicolini, Ariana M.
AU - Toth, Tyler D.
AU - Yoon, Jeong-Yeol
PY - 2016/9/1
Y1 - 2016/9/1
N2 - This work presents a novel technique using a reverse potential electrospinning mode for fabricating nanoparticle-embedded composites that can be tailored to represent various fiber diameters, surface morphologies, and functional groups necessary for improved cellular adhesion. Polycaprolactone (PCL) nanofibers were electrospun in both traditional positive (PP) and reverse potential (RP) electrical fields. The fibers were incorporated with 300 nm polystyrene (PS) fluorescent particles, which contained carboxyl, amine groups, and surfactants. In the unconventional RP, the charged colloidal particles and surfactants were shown to have an exaggerated effect on Taylor cone morphology and fiber diameter caused by the changes in charge density and surface tension of the bulk solution. The RP mode was shown to lead to a decrease in fiber diameter from 1200 ± 100 nm (diameter ± SE) for the nanofibers made with PCL alone to 440 ± 80 nm with the incorporation of colloidal particles, compared to the PP mode ranging from 530 ± 90 nm to 350 ± 50 nm, respectively. The nanoparticle-nanofiber composite substrates were cultured with human umbilical vein endothelial cells (HUVECs) and evaluated for cellular viability and adhesion for up to 5 days. Adhesion to the nanofibrous substrates was improved by 180 ± 10% with the addition of carboxylated particles and by 480 ± 60% with the functionalization of an RGD ligand compared to the PCL nanofibers. The novel approach of electrospinning in the RP mode with the addition of colloids in order to alter charge density and surface tension could be utilized towards many applications, one being implantable biomaterials and tissue engineered scaffolds as demonstrated in this work.
AB - This work presents a novel technique using a reverse potential electrospinning mode for fabricating nanoparticle-embedded composites that can be tailored to represent various fiber diameters, surface morphologies, and functional groups necessary for improved cellular adhesion. Polycaprolactone (PCL) nanofibers were electrospun in both traditional positive (PP) and reverse potential (RP) electrical fields. The fibers were incorporated with 300 nm polystyrene (PS) fluorescent particles, which contained carboxyl, amine groups, and surfactants. In the unconventional RP, the charged colloidal particles and surfactants were shown to have an exaggerated effect on Taylor cone morphology and fiber diameter caused by the changes in charge density and surface tension of the bulk solution. The RP mode was shown to lead to a decrease in fiber diameter from 1200 ± 100 nm (diameter ± SE) for the nanofibers made with PCL alone to 440 ± 80 nm with the incorporation of colloidal particles, compared to the PP mode ranging from 530 ± 90 nm to 350 ± 50 nm, respectively. The nanoparticle-nanofiber composite substrates were cultured with human umbilical vein endothelial cells (HUVECs) and evaluated for cellular viability and adhesion for up to 5 days. Adhesion to the nanofibrous substrates was improved by 180 ± 10% with the addition of carboxylated particles and by 480 ± 60% with the functionalization of an RGD ligand compared to the PCL nanofibers. The novel approach of electrospinning in the RP mode with the addition of colloids in order to alter charge density and surface tension could be utilized towards many applications, one being implantable biomaterials and tissue engineered scaffolds as demonstrated in this work.
KW - Functionalized nanoparticles
KW - Reverse potential electrospinning
KW - RGD ligand
KW - Surface tension
KW - Surfactant
UR - http://www.scopus.com/inward/record.url?scp=84973879544&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973879544&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2016.05.079
DO - 10.1016/j.colsurfb.2016.05.079
M3 - Article
C2 - 27315331
AN - SCOPUS:84973879544
VL - 145
SP - 830
EP - 838
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -