Abstract
Inter-regional signaling coordinates pattern formation in Arabidopsis thaliana embryos. However, little is known regarding the cells and molecules involved in inter-regional communication. We have characterized two related leucine-rich repeat receptor-like kinases (LRR-RLKs), RECEPTOR-LIKE PROTEIN KINASE1 (RPK1) and TOADSTOOL2 (TOAD2), which are required together for patterning the apical embryonic domain cell types that generate cotyledon primordia. Central domain protoderm patterning defects were always observed subjacent to the defective cotyledon primordia cell types in mutant embryos. In addition, RPK1-GFP and TOAD2-GFP translational fusions were both localized to the central domain protodermal cells when cotyledon primordia were first recognizable. We propose that RPK1 and TOAD2 are primarily required to maintain central domain protoderm cell fate and that the loss of this key embryonic cell type in mutant embryos results in patterning defects in other regions of the embryo including the failure to initiate cotyledon primordia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 161-170 |
| Number of pages | 10 |
| Journal | Developmental Biology |
| Volume | 314 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2008 |
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Keywords
- Arabidopsis
- Embryogenesis
- Protoderm
- Receptor-like kinases
- Signaling
ASJC Scopus subject areas
- Developmental Biology
Cite this
Two receptor-like kinases required together for the establishment of Arabidopsis cotyledon primordia. / Nodine, Michael D.; Tax, Frans.
In: Developmental Biology, Vol. 314, No. 1, 01.02.2008, p. 161-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two receptor-like kinases required together for the establishment of Arabidopsis cotyledon primordia
AU - Nodine, Michael D.
AU - Tax, Frans
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Inter-regional signaling coordinates pattern formation in Arabidopsis thaliana embryos. However, little is known regarding the cells and molecules involved in inter-regional communication. We have characterized two related leucine-rich repeat receptor-like kinases (LRR-RLKs), RECEPTOR-LIKE PROTEIN KINASE1 (RPK1) and TOADSTOOL2 (TOAD2), which are required together for patterning the apical embryonic domain cell types that generate cotyledon primordia. Central domain protoderm patterning defects were always observed subjacent to the defective cotyledon primordia cell types in mutant embryos. In addition, RPK1-GFP and TOAD2-GFP translational fusions were both localized to the central domain protodermal cells when cotyledon primordia were first recognizable. We propose that RPK1 and TOAD2 are primarily required to maintain central domain protoderm cell fate and that the loss of this key embryonic cell type in mutant embryos results in patterning defects in other regions of the embryo including the failure to initiate cotyledon primordia.
AB - Inter-regional signaling coordinates pattern formation in Arabidopsis thaliana embryos. However, little is known regarding the cells and molecules involved in inter-regional communication. We have characterized two related leucine-rich repeat receptor-like kinases (LRR-RLKs), RECEPTOR-LIKE PROTEIN KINASE1 (RPK1) and TOADSTOOL2 (TOAD2), which are required together for patterning the apical embryonic domain cell types that generate cotyledon primordia. Central domain protoderm patterning defects were always observed subjacent to the defective cotyledon primordia cell types in mutant embryos. In addition, RPK1-GFP and TOAD2-GFP translational fusions were both localized to the central domain protodermal cells when cotyledon primordia were first recognizable. We propose that RPK1 and TOAD2 are primarily required to maintain central domain protoderm cell fate and that the loss of this key embryonic cell type in mutant embryos results in patterning defects in other regions of the embryo including the failure to initiate cotyledon primordia.
KW - Arabidopsis
KW - Embryogenesis
KW - Protoderm
KW - Receptor-like kinases
KW - Signaling
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U2 - 10.1016/j.ydbio.2007.11.021
DO - 10.1016/j.ydbio.2007.11.021
M3 - Article
C2 - 18158146
AN - SCOPUS:38349162790
VL - 314
SP - 161
EP - 170
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -