TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine Bases

Anthony P. Young, Vahe Bandarian

Research output: Contribution to journalArticle

Abstract

Transfer RNA is extensively modified by the actions of a variety of enzymes. The radical S-adenosyl-L-methionine enzyme TYW1 modifies tRNAPhe forming the characteristic tricyclic ring via the condensation of carbons 2 and 3 of pyruvate. This chapter details methods that are required for studies of TYW1.

Original languageEnglish (US)
JournalMethods in Enzymology
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Keywords

  • Radical SAM
  • tRNA
  • tRNA modification
  • TYW1
  • Wyosine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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