TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine Bases

Anthony P. Young, Vahe Bandarian

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Transfer RNA is extensively modified by the actions of a variety of enzymes. The radical S-adenosyl-L-methionine enzyme TYW1 modifies tRNAPhe forming the characteristic tricyclic ring via the condensation of carbons 2 and 3 of pyruvate. This chapter details methods that are required for studies of TYW1.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsVahe Bandarian
PublisherAcademic Press Inc.
Pages119-153
Number of pages35
ISBN (Print)9780128127940
DOIs
StatePublished - 2018

Publication series

NameMethods in Enzymology
Volume606
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Radical SAM
  • TYW1
  • Wyosine
  • tRNA
  • tRNA modification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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