U1 small nuclear RNA variants differentially form ribonucleoprotein particles in vitro

Jason A. Somarelli, Annia Mesa, Carol E. Rodriguez, Shalini Sharma, Rene J. Herrera

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The U1 small nuclear (sn)RNA participates in splicing of pre-mRNAs by recognizing and binding to 5' splice sites at exon/intron boundaries. U1 snRNAs associate with 5' splice sites in the form of ribonucleoprotein particles (snRNPs) that are comprised of the U1 snRNA and 10 core components, including U1A, U1-70K, U1C and the 'Smith antigen', or Sm, heptamer. The U1 snRNA is highly conserved across a wide range of taxa; however, a number of reports have identified the presence of expressed U1-like snRNAs in multiple species, including humans. While numerous U1-like molecules have been shown to be expressed, it is unclear whether these variant snRNAs have the capacity to form snRNPs and participate in splicing. The purpose of the present study was to further characterize biochemically the ability of previously identified human U1-like variants to form snRNPs and bind to U1 snRNP proteins. A bioinformatics analysis provided support for the existence of multiple expressed variants. In vitro gel shift assays, competition assays, and immunoprecipitations (IPs) revealed that the variants formed high molecular weight assemblies to varying degrees and associated with core U1 snRNP proteins to a lesser extent than the canonical U1 snRNA. Together, these data suggest that the human U1 snRNA variants analyzed here are unable to efficiently bind U1 snRNP proteins. The current work provides additional biochemical insights into the ability of the variants to assemble into snRNPs.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalGene
Volume540
Issue number1
DOIs
StatePublished - Apr 25 2014

Fingerprint

Ribonucleoproteins
U1 Small Nuclear Ribonucleoproteins
RNA Splice Sites
snRNP Core Proteins
RNA Precursors
Computational Biology
Immunoprecipitation
Introns
Exons
Proteins
Molecular Weight
Gels
In Vitro Techniques
U1 small nuclear RNA
Antigens

Keywords

  • Sm proteins
  • Spliceosome
  • Splicing
  • U1-70K protein
  • U1A protein

ASJC Scopus subject areas

  • Genetics

Cite this

U1 small nuclear RNA variants differentially form ribonucleoprotein particles in vitro. / Somarelli, Jason A.; Mesa, Annia; Rodriguez, Carol E.; Sharma, Shalini; Herrera, Rene J.

In: Gene, Vol. 540, No. 1, 25.04.2014, p. 11-15.

Research output: Contribution to journalArticle

Somarelli, Jason A. ; Mesa, Annia ; Rodriguez, Carol E. ; Sharma, Shalini ; Herrera, Rene J. / U1 small nuclear RNA variants differentially form ribonucleoprotein particles in vitro. In: Gene. 2014 ; Vol. 540, No. 1. pp. 11-15.
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