UBIQUITIN-SPECIFIC PROTEASE16 modulates salt tolerance in arabidopsis by regulating Na+/H+ antiport activity and serine hydroxymethyltransferase stability

Huapeng Zhou, Jinfeng Zhao, Yongqing Yang, Changxi Chen, Yanfen Liu, Xuehua Jin, Limei Chen, Xueyong Li, Xing Wang Deng, Karen S Schumaker, Yan Guo

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Abstract

Protein ubiquitination is a reversible process catalyzed by ubiquitin ligases and ubiquitin-specific proteases (UBPs). We report the identification and characterization of UBP16 in Arabidopsis thaliana. UBP16 is a functional ubiquitin-specific protease and its enzyme activity is required for salt tolerance. Plants lacking UBP16 were hypersensitive to salt stress and accumulated more sodium and less potassium. UBP16 positively regulated plasma membrane Na+/H+ antiport activity. Through yeast two-hybrid screening, we identified a putative target of UBP16, SERINE HYDROXYMETHYLTRANSFERASE1 (SHM1), which has previously been reported to be involved in photorespiration and salt tolerance in Arabidopsis. We found that SHM1 is degraded in a 26S proteasome-dependent process, and UBP16 stabilizes SHM1 by removing the conjugated ubiquitin. Ser hydroxymethyltransferase activity is lower in the ubp16 mutant than in the wild type but higher than in the shm1 mutant. During salt stress, UBP16 and SHM1 function in preventing cell death and reducing reactive oxygen species accumulation, activities that are correlated with increasing Na+/H+ antiport activity. Overexpression of SHM1 in the ubp16 mutant partially rescues its salt-sensitive phenotype. Taken together, our results suggest that UBP16 is involved in salt tolerance in Arabidopsis by modulating sodium transport activity and repressing cell death at least partially through modulating SMH1stability and activity.

Original languageEnglish (US)
Pages (from-to)5106-5122
Number of pages17
JournalPlant Cell
Volume24
Issue number12
DOIs
StatePublished - Dec 2013

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Glycine Hydroxymethyltransferase
glycine hydroxymethyltransferase
Salt-Tolerance
Ion Transport
Ubiquitin
salt tolerance
Arabidopsis
serine
Serine
ubiquitin
Ubiquitin-Specific Proteases
Salts
mutants
salt stress
cell death
Cell Death
proteinases
Hydroxymethyl and Formyl Transferases
Sodium
sodium

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

UBIQUITIN-SPECIFIC PROTEASE16 modulates salt tolerance in arabidopsis by regulating Na+/H+ antiport activity and serine hydroxymethyltransferase stability. / Zhou, Huapeng; Zhao, Jinfeng; Yang, Yongqing; Chen, Changxi; Liu, Yanfen; Jin, Xuehua; Chen, Limei; Li, Xueyong; Deng, Xing Wang; Schumaker, Karen S; Guo, Yan.

In: Plant Cell, Vol. 24, No. 12, 12.2013, p. 5106-5122.

Research output: Contribution to journalArticle

Zhou, Huapeng ; Zhao, Jinfeng ; Yang, Yongqing ; Chen, Changxi ; Liu, Yanfen ; Jin, Xuehua ; Chen, Limei ; Li, Xueyong ; Deng, Xing Wang ; Schumaker, Karen S ; Guo, Yan. / UBIQUITIN-SPECIFIC PROTEASE16 modulates salt tolerance in arabidopsis by regulating Na+/H+ antiport activity and serine hydroxymethyltransferase stability. In: Plant Cell. 2013 ; Vol. 24, No. 12. pp. 5106-5122.
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