Ultra-slim plastic endomicroscope objective for non-linear microscopy

Matthew Kyrish, Urs Utzinger, Michael R. Descour, Brenda K. Baggett, Tomasz S. Tkaczy

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Non-linear microscopy has the potential to provide clinically useful information on the structure of biological tissue in vivo via an endomicroscope. The ability to use plastic as the optical material in a multiphoton objective was evaluated based on several criteria including autofluorescence, injection molding induced birefringence, and pulse broadening due to group velocity dispersion. An all-plastic, refractive ultraslim endoscope objective was built with design specifications of NA = 0.4, FOV = 250 μm, 1.27 mm outer diameter, and 0.8 mm clear aperture. Initial images of second-harmonic generation signal (illumination at 780 nm) in collagen fibers and two-photon excited fluorescence (illumination at 920 nm) of Convallaria rhizome are reported.

Original languageEnglish (US)
Pages (from-to)7603-7615
Number of pages13
JournalOptics Express
Volume19
Issue number8
DOIs
StatePublished - Apr 11 2011

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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    Kyrish, M., Utzinger, U., Descour, M. R., Baggett, B. K., & Tkaczy, T. S. (2011). Ultra-slim plastic endomicroscope objective for non-linear microscopy. Optics Express, 19(8), 7603-7615. https://doi.org/10.1364/OE.19.007603