Ultrasonic enhancement of αvβ3 expressing-cells with targeted contrast agents

Paul A. Dayton, David Pearson, Jarrod Clark, Scott Simon, Patricia Schumann, Reena Zutshi, Terry O Matsunaga, Katherine W. Ferrara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Targeted contrast agents have the potential to substantially improve the ability of ultrasound contrast agents to delineate otherwise difficult-to-detect pathologies. In this manuscript, optical microscopy and acoustic backscatter measurements from human umbilical vein endothelial cells (HUVEC) exposed to targeted and control microbubbles illustrate the high affinity of targeted agents for the αvβ3 integrin. Backscatter measurements are provided for cell layers exposed to three types of targeted contrast agents in addition to two controls. Acoustic studies illustrate a backscatter amplitude increase from αvβ3 expressing cells exposed to the targeted contrast agent of up to 13 fold compared to non-targeted agents. Additionally, echo decorrelation is shown to be a function of acoustic pressure and microbubble density. Frequency domain analysis demonstrates that adherent targeted microbubbles exhibit a significantly more narrowband spectrum than free microbubbles. These results suggest that targeted contrast agents may provide additional sensitivity in the detection of tumors, and that adherent contrast agents may be differentiable from free-floating microbubbles.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE Ultrasonics Symposium
EditorsD.E. Yuhas, S.C. Schneider
Pages540-543
Number of pages4
Volume1
Publication statusPublished - 2003
Externally publishedYes
Event2003 IEEE Ultrasonics Symposium - Proceedings - Honolulu, HI, United States
Duration: Oct 5 2003Oct 8 2003

Other

Other2003 IEEE Ultrasonics Symposium - Proceedings
CountryUnited States
CityHonolulu, HI
Period10/5/0310/8/03

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ASJC Scopus subject areas

  • Engineering(all)

Cite this

Dayton, P. A., Pearson, D., Clark, J., Simon, S., Schumann, P., Zutshi, R., ... Ferrara, K. W. (2003). Ultrasonic enhancement of αvβ3 expressing-cells with targeted contrast agents. In D. E. Yuhas, & S. C. Schneider (Eds.), Proceedings of the IEEE Ultrasonics Symposium (Vol. 1, pp. 540-543)