Ultrasound-mediated transfection of canine myocardium by intravenous administration of cationic microbubble-linked plasmid DNA

Mani Vannan, Thomas McCreery, Peng Li, Zhenguo Han, Evan Unger, Bettina Kuersten, Elizabeth Nabel, Sanjay Rajagopalan

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

We tested the hypothesis that targeted disruption of cationic microbubble-linked plasmid DNA, using diagnostic ultrasound, may aid transfection of large animal myocardium. Plasmid DNA encoding for CAT (pCAT, chloramphenicol acetyltransferase) was bound to a novel cationic microbubble containing MRX-225 for intravenous administration, and 16 dogs in 4 groups variously received this conjugate or plasmid only, or were exposed to ultrasound. Histochemical staining and enzyme-linked immunosorbent assay analysis showed CAT activity in the myocardium of only those animals that received microbubble-linked DNA and were exposed to ultrasound. Thus, disruption of cationic-linked, low-dose plasmid systems by diagnostic ultrasound may facilitate transfection of large annual hearts.

Original languageEnglish (US)
Pages (from-to)214-218
Number of pages5
JournalJournal of the American Society of Echocardiography
Volume15
Issue number3
DOIs
StatePublished - Mar 2002

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ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

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