Ultrasound-mediated transfection of canine myocardium by intravenous administration of cationic microbubble-linked plasmid DNA

Mani Vannan, Thomas McCreery, Peng Li, Zhenguo Han, Evan C Unger, Bettina Kuersten, Elizabeth Nabel, Sanjay Rajagopalan

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

We tested the hypothesis that targeted disruption of cationic microbubble-linked plasmid DNA, using diagnostic ultrasound, may aid transfection of large animal myocardium. Plasmid DNA encoding for CAT (pCAT, chloramphenicol acetyltransferase) was bound to a novel cationic microbubble containing MRX-225 for intravenous administration, and 16 dogs in 4 groups variously received this conjugate or plasmid only, or were exposed to ultrasound. Histochemical staining and enzyme-linked immunosorbent assay analysis showed CAT activity in the myocardium of only those animals that received microbubble-linked DNA and were exposed to ultrasound. Thus, disruption of cationic-linked, low-dose plasmid systems by diagnostic ultrasound may facilitate transfection of large annual hearts.

Original languageEnglish (US)
Pages (from-to)214-218
Number of pages5
JournalJournal of the American Society of Echocardiography
Volume15
Issue number3
DOIs
StatePublished - Mar 2002

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Microbubbles
Intravenous Administration
Transfection
Canidae
Myocardium
Plasmids
Chloramphenicol O-Acetyltransferase
DNA
Ultrasonography
Enzyme-Linked Immunosorbent Assay
Dogs
Staining and Labeling

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Ultrasound-mediated transfection of canine myocardium by intravenous administration of cationic microbubble-linked plasmid DNA. / Vannan, Mani; McCreery, Thomas; Li, Peng; Han, Zhenguo; Unger, Evan C; Kuersten, Bettina; Nabel, Elizabeth; Rajagopalan, Sanjay.

In: Journal of the American Society of Echocardiography, Vol. 15, No. 3, 03.2002, p. 214-218.

Research output: Contribution to journalArticle

Vannan, Mani ; McCreery, Thomas ; Li, Peng ; Han, Zhenguo ; Unger, Evan C ; Kuersten, Bettina ; Nabel, Elizabeth ; Rajagopalan, Sanjay. / Ultrasound-mediated transfection of canine myocardium by intravenous administration of cationic microbubble-linked plasmid DNA. In: Journal of the American Society of Echocardiography. 2002 ; Vol. 15, No. 3. pp. 214-218.
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