Ultrastructure of the replication site in Taura syndrome virus (TSV)-infected cells

Thinnarat Srisuvan, Carlos R Pantoja-Morales, Rita M. Redman, Donald V Lightner

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Taura syndrome virus (TSV) is a member of the family Dicistroviridae that infects Pacific white shrimp Litopenaeus vannamei (also called Penaeus vannamei), and its replication strategy is largely unknown. To identify the viral replication site within infected shrimp cells, the viral RNA was located in correlation with virus-induced membrane rearrangement. Ultrastructural changes in the infected cells, analyzed by transmission electron microscopy (TEM), included the induction and proliferation of intracellular vesicle-like membranes, while the intracytoplasmic inclusion bodies and pyknotic nuclei indicative of TSV infection were frequently seen. TSV plus-strand RNA, localized by electron microscopic in situ hybridization (EM-ISH) using TSV-specific cDNA probes, was found to be associated with the membranous structures. Moreover, TSV particles were observed in infected cells by TEM, and following EM-ISH, they were also seen in close association with the proliferating membranes. Taken together, our results suggest that the membranous vesicle-like structures carry the TSV RNA replication complex and that they are the site of nascent viral RNA synthesis. Further investigations on cellular origins and biochemical compositions of these membranous structures will elucidate the morphogenesis and propagation strategy of TSV.

Original languageEnglish (US)
Pages (from-to)89-101
Number of pages13
JournalDiseases of Aquatic Organisms
Volume73
Issue number2
StatePublished - Dec 14 2006

Fingerprint

Taura syndrome virus
ultrastructure
virus
RNA
cells
Litopenaeus vannamei
membrane
vesicle
in situ hybridization
transmission electron microscopy
Dicistroviridae
shrimp
electrons
virus particle
electron
biochemical composition
morphogenesis
inclusion bodies
virus replication
virion

Keywords

  • In situ hybridization
  • Litopenaeus vannamei
  • Taura syndrome virus
  • Transmission electron microscopy
  • TSV

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Ultrastructure of the replication site in Taura syndrome virus (TSV)-infected cells. / Srisuvan, Thinnarat; Pantoja-Morales, Carlos R; Redman, Rita M.; Lightner, Donald V.

In: Diseases of Aquatic Organisms, Vol. 73, No. 2, 14.12.2006, p. 89-101.

Research output: Contribution to journalArticle

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