Ultrastructure of tight junctions in prostaglandin-exposed rat stomach

Ronald S Weinstein, Barbara F. Banner, Jerome R. Kuszak, Neil J. Thomas, Bendicht U. Pauli

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of intragastric misoprostol, a synthetic prostaglandin E1 methyl ester analog, on the morphology of rat stomach was studied by transmission and scanning electron microscopy. Adult male Sprague-Dawley rats received misoprostol at dose levels ranging from 50 to 1000 μg/kg body weight and were sacrificed after 30 min. At all dose levels, normal organelle ultrastructure was maintained in epithelial cells on the surface, within gastric pits, and lining the gastric glands. Quantitative freeze-fracture electron microscopy was used to examine the influence of misoprostol on epithelial-cell tight junctions, major structural components of the mucosal barrier. The numbers of intramembrane fibrils and the depths of the tight-junction complexes did not differ significantly between controls and animals exposed to 1000 μg/kg misoprostol (P<0.05). Therefore, alterations in gastric epithelial tight-junction ultrastructure do not appear to account for the cytoprotective effect of misoprostol.

Original languageEnglish (US)
JournalDigestive Diseases and Sciences
Volume31
Issue number2 Supplement
DOIs
StatePublished - Feb 1986
Externally publishedYes

Fingerprint

Misoprostol
Tight Junctions
Prostaglandins
Stomach
Epithelial Cells
Synthetic Prostaglandins
Scanning Transmission Electron Microscopy
Intercellular Junctions
Gastric Mucosa
Organelles
Sprague Dawley Rats
Electron Microscopy
Body Weight

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Ultrastructure of tight junctions in prostaglandin-exposed rat stomach. / Weinstein, Ronald S; Banner, Barbara F.; Kuszak, Jerome R.; Thomas, Neil J.; Pauli, Bendicht U.

In: Digestive Diseases and Sciences, Vol. 31, No. 2 Supplement, 02.1986.

Research output: Contribution to journalArticle

Weinstein, Ronald S ; Banner, Barbara F. ; Kuszak, Jerome R. ; Thomas, Neil J. ; Pauli, Bendicht U. / Ultrastructure of tight junctions in prostaglandin-exposed rat stomach. In: Digestive Diseases and Sciences. 1986 ; Vol. 31, No. 2 Supplement.
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