Uncoupling of cardiac cells by fatty acids: Structure-activity relationships

Janis M Burt, K. D. Massey, B. N. Minnich

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The permeability and conductance of gap junctions between pairs of neonatal rat heart cells were rapidly and reversibly decreased by oleic acid in a dose- and time-dependent manner. Other unsaturated fatty acids (C-18: cis 6, 9, or 11, and C-18, 16, and 14, cis 9), saturated fatty acids (C-10, 12, and 14), and saturated fatty alcohols (C-8, 10, and 12) also caused uncoupling. The most effective compounds of the unsaturated and saturated fatty acid and saturated fatty alcohol series caused essentially complete uncoupling at comparable aqueous concentrations. However, oleic acid uncoupled cells at membrane concentrations as low as 1 mol%, whereas decanoic acid required upwards of 35 mol%. The channels that support the action potential remained functional at these same membrane concentrations. The data are discussed in terms of the possible mechanism by which these compounds cause uncoupling and the possible role of uncoupling by nonesterified free fatty acids in the initiation of arrhythmias during and after ischemic insults.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume260
Issue number3 29-3
StatePublished - 1991

Fingerprint

Fatty Alcohols
Oleic Acid
Structure-Activity Relationship
Unsaturated Fatty Acids
Nonesterified Fatty Acids
Fatty Acids
Membranes
Gap Junctions
Action Potentials
Rats
Cardiac Arrhythmias
Permeability
Cell Membrane
decanoic acid

Keywords

  • Arrhythmias
  • Dysrhytmias
  • Gap junctions
  • Intercellular communication
  • Myocardial ischemia
  • Nonesterified free fatty acids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Uncoupling of cardiac cells by fatty acids : Structure-activity relationships. / Burt, Janis M; Massey, K. D.; Minnich, B. N.

In: American Journal of Physiology - Cell Physiology, Vol. 260, No. 3 29-3, 1991.

Research output: Contribution to journalArticle

@article{71907b74ca9c45928ea2cebece7d3fbb,
title = "Uncoupling of cardiac cells by fatty acids: Structure-activity relationships",
abstract = "The permeability and conductance of gap junctions between pairs of neonatal rat heart cells were rapidly and reversibly decreased by oleic acid in a dose- and time-dependent manner. Other unsaturated fatty acids (C-18: cis 6, 9, or 11, and C-18, 16, and 14, cis 9), saturated fatty acids (C-10, 12, and 14), and saturated fatty alcohols (C-8, 10, and 12) also caused uncoupling. The most effective compounds of the unsaturated and saturated fatty acid and saturated fatty alcohol series caused essentially complete uncoupling at comparable aqueous concentrations. However, oleic acid uncoupled cells at membrane concentrations as low as 1 mol{\%}, whereas decanoic acid required upwards of 35 mol{\%}. The channels that support the action potential remained functional at these same membrane concentrations. The data are discussed in terms of the possible mechanism by which these compounds cause uncoupling and the possible role of uncoupling by nonesterified free fatty acids in the initiation of arrhythmias during and after ischemic insults.",
keywords = "Arrhythmias, Dysrhytmias, Gap junctions, Intercellular communication, Myocardial ischemia, Nonesterified free fatty acids",
author = "Burt, {Janis M} and Massey, {K. D.} and Minnich, {B. N.}",
year = "1991",
language = "English (US)",
volume = "260",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "3 29-3",

}

TY - JOUR

T1 - Uncoupling of cardiac cells by fatty acids

T2 - Structure-activity relationships

AU - Burt, Janis M

AU - Massey, K. D.

AU - Minnich, B. N.

PY - 1991

Y1 - 1991

N2 - The permeability and conductance of gap junctions between pairs of neonatal rat heart cells were rapidly and reversibly decreased by oleic acid in a dose- and time-dependent manner. Other unsaturated fatty acids (C-18: cis 6, 9, or 11, and C-18, 16, and 14, cis 9), saturated fatty acids (C-10, 12, and 14), and saturated fatty alcohols (C-8, 10, and 12) also caused uncoupling. The most effective compounds of the unsaturated and saturated fatty acid and saturated fatty alcohol series caused essentially complete uncoupling at comparable aqueous concentrations. However, oleic acid uncoupled cells at membrane concentrations as low as 1 mol%, whereas decanoic acid required upwards of 35 mol%. The channels that support the action potential remained functional at these same membrane concentrations. The data are discussed in terms of the possible mechanism by which these compounds cause uncoupling and the possible role of uncoupling by nonesterified free fatty acids in the initiation of arrhythmias during and after ischemic insults.

AB - The permeability and conductance of gap junctions between pairs of neonatal rat heart cells were rapidly and reversibly decreased by oleic acid in a dose- and time-dependent manner. Other unsaturated fatty acids (C-18: cis 6, 9, or 11, and C-18, 16, and 14, cis 9), saturated fatty acids (C-10, 12, and 14), and saturated fatty alcohols (C-8, 10, and 12) also caused uncoupling. The most effective compounds of the unsaturated and saturated fatty acid and saturated fatty alcohol series caused essentially complete uncoupling at comparable aqueous concentrations. However, oleic acid uncoupled cells at membrane concentrations as low as 1 mol%, whereas decanoic acid required upwards of 35 mol%. The channels that support the action potential remained functional at these same membrane concentrations. The data are discussed in terms of the possible mechanism by which these compounds cause uncoupling and the possible role of uncoupling by nonesterified free fatty acids in the initiation of arrhythmias during and after ischemic insults.

KW - Arrhythmias

KW - Dysrhytmias

KW - Gap junctions

KW - Intercellular communication

KW - Myocardial ischemia

KW - Nonesterified free fatty acids

UR - http://www.scopus.com/inward/record.url?scp=0025752525&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025752525&partnerID=8YFLogxK

M3 - Article

C2 - 2003571

AN - SCOPUS:0025752525

VL - 260

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 3 29-3

ER -