Uncoupling the functions of a multifunctional protein: The isolation of a DNA pilot protein mutant that affects particle morphogenesis

James E. Cherwa, Lindsey N. Young, Bentley A Fane

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Defective øX174 H protein-mediated DNA piloting indirectly influences the entire viral lifecycle. Faulty piloting can mask the H protein's other functions or inefficient penetration may be used to explain defects in post-piloting phenomena. For example, optimal synthesis of other viral proteins requires de novo H protein biosynthesis. As low protein concentrations affect morphogenesis, protein H's assembly functions remain obscure. An H protein mutant was isolated that allowed morphogenetic effects to be characterized independent of its other functions. The mutant protein aggregates assembly intermediates. Although excess internal scaffolding protein restores capsid assembly, the resulting mutant H protein-containing particles are less infectious. In addition, nonviable phenotypes of am(H) mutants in Su+ hosts, which insert non-wild-type amino acids, do not always correlate with a lack of missense protein function. Phenotypes are highly influenced by host and phage physiology. This phenomenon was unique to am(H) mutants, not observed with amber mutants in other genes.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalVirology
Volume411
Issue number1
DOIs
StatePublished - Mar 1 2011

Fingerprint

Mutant Proteins
Morphogenesis
DNA
Proteins
Amber
Phenotype
Capsid Proteins
Protein Biosynthesis
Viral Proteins
Masks
Bacteriophages
Amino Acids
Genes

Keywords

  • ØX174
  • DNA pilot protein
  • Scaffolding protein
  • Virus assembly

ASJC Scopus subject areas

  • Virology

Cite this

Uncoupling the functions of a multifunctional protein : The isolation of a DNA pilot protein mutant that affects particle morphogenesis. / Cherwa, James E.; Young, Lindsey N.; Fane, Bentley A.

In: Virology, Vol. 411, No. 1, 01.03.2011, p. 9-14.

Research output: Contribution to journalArticle

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