Uncovering of intracellular water in cultured cells

Jean Philippe Galons, Silvia Lope-Piedrafita, Joseph L. Divijak, Curt Corum, Robert J. Gillies, Theodore P. Trouard

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The complexity of biologic tissues, with multiple compartments each with its own diffusion and relaxation properties, requires complex formalisms to model water signal in most magnetic resonance imaging or magnetic resonance spectroscopy experiments. In this article, we describe a magnetic susceptibility-induced shift in the resonance frequency of extracellular water by the introduction of a gadolinium contrast agent to medium perfusing a hollow fiber bioreactor. The frequency shift of the extracellular water (+185 Hz at 9.4 T) uncovers the intracellular water and allows direct measurement of motional and relaxation properties of the intracellular space. The proposed method provides a unique tool for understanding the mechanisms underlining diffusion and relaxation in the intracellular space.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalMagnetic Resonance in Medicine
Volume54
Issue number1
DOIs
StatePublished - Jul 1 2005

Keywords

  • Bioreactor
  • Bulk susceptibility
  • Diffusion
  • Intracellular water
  • Relaxation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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