Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: Modeling, measurements, and implications

Rebekah Drezek, Konstantin Sokolov, Urs Utzinger, Iouri Boiko, Anais Malpica, Michele Follen, Rebecca Richards-Kortum

Research output: Contribution to journalArticle

262 Scopus citations

Abstract

Objective: At 380 nm excitation, cervical tissue fluorescence spectra demonstrate characteristic changes with both patient age and the presence of dysplasia. A Monte Carlo model was developed in order to quantitatively examine how intrinsic NADH and collagen fluorescence, in combination with tissue scattering and absorption properties, yield measured tissue spectra. Methods: Excitation-emission matrices were measured for live cervical cells and collagen gel phantoms. Fluorescence microscopy of fresh tissue sections was performed to obtain the location and density of fluorophores as a function of patient age and the presence of dysplasia. A Monte Carlo model was developed which incorporated measurements of fluorophore line shapes and spatial distributions. Results: Modeled spectra were consistent with clinical measurements and indicate that an increase in NADH fluorescence and decrease in collagen fluorescence create clinically observed differences between normal and dysplastic tissue spectra. Model predictions were most sensitive to patient age and epithelial thickness. Conclusions: Monte Carlo techniques provide an important means to investigate the combined contributions of multiple fluorophores to measured emission spectra. The approach will prove increasingly valuable as a more sophisticated understanding of in vivo optical properties is developed.

Original languageEnglish (US)
Pages (from-to)385-396
Number of pages12
JournalJournal of biomedical optics
Volume6
Issue number4
DOIs
StatePublished - Oct 2001

Keywords

  • Cervix
  • Fluorescence
  • Light propagation
  • Monte Carlo

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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