Unstirred Water Layers and the Kinetics of Organic Cation Transport

Takahiro Shibayama, Mark Morales, Xiaohong Zhang, Lucy J. Martínez-Guerrero, Alfred Berteloot, Timothy W Secomb, Stephen Wright

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Abstract Purpose: Unstirred water layers (UWLs) present an unavoidable complication to the measurement of transport kinetics in cultured cells, and the high rates of transport achieved by overexpressing heterologous transporters exacerbate the UWL effect. This study examined the correlation between measured J<inf>max</inf> and K<inf>t</inf> values and the effect of manipulating UWL thickness or transport J<inf>max</inf> on the accuracy of experimentally determined kinetics of the multidrug transporters, OCT2 and MATE1. Methods: Transport of TEA and MPP was measured in CHO cells that stably expressed human OCT2 or MATE1. UWL thickness was manipulated by vigorous reciprocal shaking. Several methods were used to manipulate maximal transport rates. Results: Vigorous stirring stimulated uptake of OCT2-mediated transport by decreasing apparent K<inf>t</inf> (K<inf>tapp</inf>) values. Systematic reduction in transport rates was correlated with reduction in K<inf>tapp</inf> values. The slope of these relationships indicated a 1500Â

Original languageEnglish (US)
Article number1675
Pages (from-to)2937-2949
Number of pages13
JournalPharmaceutical Research
Volume32
Issue number9
DOIs
StatePublished - Sep 8 2015

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Cations
Kinetics
Water
CHO Cells
Cultured Cells
Cells

Keywords

  • kinetics
  • organic cation
  • transport
  • unstirred water layers

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Organic Chemistry
  • Molecular Medicine
  • Pharmacology (medical)
  • Biotechnology
  • Pharmacology

Cite this

Shibayama, T., Morales, M., Zhang, X., Martínez-Guerrero, L. J., Berteloot, A., Secomb, T. W., & Wright, S. (2015). Unstirred Water Layers and the Kinetics of Organic Cation Transport. Pharmaceutical Research, 32(9), 2937-2949. [1675]. https://doi.org/10.1007/s11095-015-1675-5

Unstirred Water Layers and the Kinetics of Organic Cation Transport. / Shibayama, Takahiro; Morales, Mark; Zhang, Xiaohong; Martínez-Guerrero, Lucy J.; Berteloot, Alfred; Secomb, Timothy W; Wright, Stephen.

In: Pharmaceutical Research, Vol. 32, No. 9, 1675, 08.09.2015, p. 2937-2949.

Research output: Contribution to journalArticle

Shibayama, T, Morales, M, Zhang, X, Martínez-Guerrero, LJ, Berteloot, A, Secomb, TW & Wright, S 2015, 'Unstirred Water Layers and the Kinetics of Organic Cation Transport', Pharmaceutical Research, vol. 32, no. 9, 1675, pp. 2937-2949. https://doi.org/10.1007/s11095-015-1675-5
Shibayama T, Morales M, Zhang X, Martínez-Guerrero LJ, Berteloot A, Secomb TW et al. Unstirred Water Layers and the Kinetics of Organic Cation Transport. Pharmaceutical Research. 2015 Sep 8;32(9):2937-2949. 1675. https://doi.org/10.1007/s11095-015-1675-5
Shibayama, Takahiro ; Morales, Mark ; Zhang, Xiaohong ; Martínez-Guerrero, Lucy J. ; Berteloot, Alfred ; Secomb, Timothy W ; Wright, Stephen. / Unstirred Water Layers and the Kinetics of Organic Cation Transport. In: Pharmaceutical Research. 2015 ; Vol. 32, No. 9. pp. 2937-2949.
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