Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model

Xuelian Lei, Lin Cai, Xiao Li, Hua Xu, Chong Geng, Chunhui Wang

Research output: Contribution to journalArticle

Abstract

Na+/H+ exchangers (NHEs) have been shown to be involved in regulating cell volume and maintaining fluid and electrolyte homeostasis. Pooled evidences have suggested that loss of Na+/H+ exchanger isoform 8 (NHE8) impairs intestinal mucosa. Whether NHE8 participates in the pathology of infectious colitis is still unknown. Our previous study demonstrated that somatostatin (SST) could stimulate the expression of intestinal NHE8 so as to facilitate Na+ absorption under normal condition. This study further explored whether NHE8 participates in the pathological processes of infectious colitis and the effects of SST on intestinal NHE8 expression in the setting of infectious colitis. Our data showed that NHE8 expression was reduced in Citrobacter rodentium (CR) infected mice. Up-regulation of NHE8 improved diarrhea symptom and mucosal damage induced by CR. In vitro, a similar observation was also seen in Enteropathogenic E. coli (EPEC) infected Caco-2 cells. Seglitide, a SST receptor (SSTR) 2 agonist, partly reversed the inhibiting action of EPEC on NHE8 expression, but SSTR5 agonist (L-817,818) had no effect on the expression of NHE8. Moreover, SST blocked the phosphorylation of p38 in EPEC-infected Caco-2 cells. Taken together, these results suggest that enhancement of intestinal NHE8 expression by SST could ameliorate the symptoms of mice with infectious colitis.

Original languageEnglish (US)
Pages (from-to)269-275
Number of pages7
JournalKorean Journal of Physiology and Pharmacology
Volume22
Issue number3
DOIs
StatePublished - May 1 2018

Fingerprint

Sodium-Hydrogen Antiporter
Colitis
Somatostatin
Diarrhea
Protein Isoforms
Up-Regulation
Enteropathogenic Escherichia coli
Citrobacter rodentium
Caco-2 Cells
seglitide
Pathologic Processes
Intestinal Mucosa
Cell Size
Electrolytes
Homeostasis
Phosphorylation
Pathology

Keywords

  • Anti-inflammatory agents
  • Colitis
  • Diarrhea
  • NHE8 protein
  • Somatostatin

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model. / Lei, Xuelian; Cai, Lin; Li, Xiao; Xu, Hua; Geng, Chong; Wang, Chunhui.

In: Korean Journal of Physiology and Pharmacology, Vol. 22, No. 3, 01.05.2018, p. 269-275.

Research output: Contribution to journalArticle

Lei, Xuelian ; Cai, Lin ; Li, Xiao ; Xu, Hua ; Geng, Chong ; Wang, Chunhui. / Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model. In: Korean Journal of Physiology and Pharmacology. 2018 ; Vol. 22, No. 3. pp. 269-275.
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