Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

Katsuyuki Nakano, Terutoshi Nakao, Karl H. Schram, William M. Hammargren, Thomas D. McClure, Michael Katz, Esklid Petersen

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.

Original languageEnglish (US)
Pages (from-to)169-183
Number of pages15
JournalClinica Chimica Acta
Volume218
Issue number2
DOIs
StatePublished - Sep 30 1993

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS'. Together they form a unique fingerprint.

  • Cite this