Use of integrated cell culture-PCR to evaluate the effectiveness of poliovirus inactivation by chlorine

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42 Citations (Scopus)

Abstract

Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.

Original languageEnglish (US)
Pages (from-to)2267-2268
Number of pages2
JournalApplied and Environmental Microbiology
Volume66
Issue number5
DOIs
StatePublished - May 2000

Fingerprint

Enterovirus C
Poliovirus
Chlorine
chlorine
inactivation
cell culture
Cell Culture Techniques
Polymerase Chain Reaction
Disinfection
disinfection
virus
assay
Viruses
viruses
assays

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

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abstract = "Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.",
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