Use of non-radioactively labeled DNA probes for the detection of a baculovirus from Penaeus monodon by in situ hybridization on fixed tissue

Bonnie T. Poulos, Jocelyne Mari, Jean Robert Bonami, Rita Redman, Donald V Lightner

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Clones isolated from a genomic library of the baculovirus PmSNPV (= MBV) were used to prepare DNA probes for detection of PmSNPV in shrimp tissue. Davidson's AFA fixed shrimp tissues were probed using DNA that had been labeled with the hapten, digoxigenin, by an in situ hybridization assay. The DNA probes readily distinguished PmSNPV-infected from uninfected shrimp tissue. The reactions were localized to the hepatopancreas and midgut, the sites where this virus is detected by standard histological methods. The probes did not react to shrimp infected with Baculovirus penaei (BP = PvSNPV).

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalJournal of Virological Methods
Volume49
Issue number2
DOIs
StatePublished - 1994

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Penaeidae
Baculoviridae
DNA Probes
In Situ Hybridization
Hepatopancreas
Digoxigenin
Genomic Library
Haptens
Clone Cells
Viruses
DNA

Keywords

  • DNA probe
  • In situ hybridization
  • MBV
  • PmSNPV
  • Shrimp baculovirus

ASJC Scopus subject areas

  • Virology

Cite this

Use of non-radioactively labeled DNA probes for the detection of a baculovirus from Penaeus monodon by in situ hybridization on fixed tissue. / Poulos, Bonnie T.; Mari, Jocelyne; Bonami, Jean Robert; Redman, Rita; Lightner, Donald V.

In: Journal of Virological Methods, Vol. 49, No. 2, 1994, p. 187-193.

Research output: Contribution to journalArticle

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abstract = "Clones isolated from a genomic library of the baculovirus PmSNPV (= MBV) were used to prepare DNA probes for detection of PmSNPV in shrimp tissue. Davidson's AFA fixed shrimp tissues were probed using DNA that had been labeled with the hapten, digoxigenin, by an in situ hybridization assay. The DNA probes readily distinguished PmSNPV-infected from uninfected shrimp tissue. The reactions were localized to the hepatopancreas and midgut, the sites where this virus is detected by standard histological methods. The probes did not react to shrimp infected with Baculovirus penaei (BP = PvSNPV).",
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AU - Redman, Rita

AU - Lightner, Donald V

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