Use of rapid genomic deletion typing to monitor a tuberculosis outbreak within an urban homeless population

Robert Freeman, Midori Kato-Maeda, Kirsten A. Hauge, Kathleen L. Horan, Eyal - Oren, Masahiro Narita, Carolyn K. Wallis, Don Cave, Charles M. Nolan, Peter M. Small, Gerard A. Cangelosi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Beginning in mid-2002, a large tuberculosis outbreak occurred among homeless persons in King County, Washington. In order to further monitor the outbreak following its peak in 2003, Mycobacterium tuberculosis isolates from all new King County tuberculosis (TB) patients in 2004 and the first half of 2005 (n = 220) were genotyped by using a rapid comparative genomics-based (genomic deletion-typing) approach, with confirmation by mycobacterial interspersed repetitive units and repetitive-sequence-based PCR (rep-PCR). Results were compared to retrospective genotypic data from 1995 to 2003. The outbreak strain SBRI9, which was not seen among King County homeless persons prior to 2002, accounted for 16 out of 30 TB cases (53%) within this population in 2002. This trend continued with 27 out of 35 cases (77%) caused by the outbreak strain in 2003, 11 out of 13 cases (85%) caused by the outbreak strain in 2004, and 4 out of 10 cases (40%) caused by the outbreak strain in the first 5 months of 2005. Thus, the outbreak strain remained well established within this homeless population throughout the study period. At least four SBRI9 cases were in people who had previously been infected by other strains. The novel PCR-based strain-typing approach used in this investigation proved to be cost-effective and very rapid. In most cases, it was possible to analyze DNA extracted directly from primary isolation (Mycobacterium growth indicator tube) cultures submitted by clinical laboratories, a feature that markedly reduced the delay between diagnosis and strain typing results. This rapid turnaround facilitated public health efforts to prevent new outbreaks involving this strain.

Original languageEnglish (US)
Pages (from-to)5550-5554
Number of pages5
JournalJournal of Clinical Microbiology
Volume43
Issue number11
DOIs
StatePublished - Nov 2005
Externally publishedYes

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Urban Population
Disease Outbreaks
Tuberculosis
Homeless Persons
Polymerase Chain Reaction
Nucleic Acid Repetitive Sequences
Mycobacterium
Genomics
Mycobacterium tuberculosis
Population
Public Health
Costs and Cost Analysis
DNA
Growth

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Use of rapid genomic deletion typing to monitor a tuberculosis outbreak within an urban homeless population. / Freeman, Robert; Kato-Maeda, Midori; Hauge, Kirsten A.; Horan, Kathleen L.; Oren, Eyal -; Narita, Masahiro; Wallis, Carolyn K.; Cave, Don; Nolan, Charles M.; Small, Peter M.; Cangelosi, Gerard A.

In: Journal of Clinical Microbiology, Vol. 43, No. 11, 11.2005, p. 5550-5554.

Research output: Contribution to journalArticle

Freeman, R, Kato-Maeda, M, Hauge, KA, Horan, KL, Oren, E, Narita, M, Wallis, CK, Cave, D, Nolan, CM, Small, PM & Cangelosi, GA 2005, 'Use of rapid genomic deletion typing to monitor a tuberculosis outbreak within an urban homeless population', Journal of Clinical Microbiology, vol. 43, no. 11, pp. 5550-5554. https://doi.org/10.1128/JCM.43.11.5550-5554.2005
Freeman, Robert ; Kato-Maeda, Midori ; Hauge, Kirsten A. ; Horan, Kathleen L. ; Oren, Eyal - ; Narita, Masahiro ; Wallis, Carolyn K. ; Cave, Don ; Nolan, Charles M. ; Small, Peter M. ; Cangelosi, Gerard A. / Use of rapid genomic deletion typing to monitor a tuberculosis outbreak within an urban homeless population. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 11. pp. 5550-5554.
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