Use of staphylococcal enterotoxin a-induced lymphoproliferation and interleukin 2 production as indicators of immunotoxicity

Lynnda L. Reid, Kenneth L. Hastings, A Jay Gandolfi, Mark Van Ert

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Suppression of mitogen-induced splenocyte lymphoproliferation and interleukin 2 (IL-2) production can be used as indicators of immunotoxicity. Staphylococcal enterotoxin A (SEA) is both a potent mitogen and the most potent in vitro inducer of IL-2 production that has been described. An in vitro system was used to measure impairment of SEA-induced lymphoproliferation and IL-2 production using splenocytes from female C57BL/6 mice dosed with either cyclosporin A (30 mg/kg/day, 14 days), benzene (220, 440, or 880 mg/kg/day, 14 days), or vehicle. Splenocytes were stimulated with either concanavalin A (con A) or SEA. Benzene- and cyclosporin A-treated mice demonstrated significant decreases in splenocyte proliferation. IL-2 production was determined by incubating splenocyte culture supernatants with IL-2 dependent cytotoxic T-cells (CTLL-2), pulsing with 3H-thymidine, and determining amount of incorporated label. Cell proliferation and IL-2 production were inhibited by both benzene and cyclosporin A, effects more clearly demonstrated using SEA than con A. SEA was a superior mitogen compared to con A in the assays evaluated here.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalDrug and Chemical Toxicology
Volume17
Issue number1
DOIs
StatePublished - 1994

Fingerprint

Enterotoxins
Interleukin-2
Benzene
Mitogens
Cyclosporine
Concanavalin A
T-cells
Cell proliferation
Cell culture
Labels
Assays
Inbred C57BL Mouse
Thymidine
Cell Proliferation
Staphylococcal enterotoxin A
T-Lymphocytes

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Chemical Health and Safety
  • Chemistry(all)

Cite this

Use of staphylococcal enterotoxin a-induced lymphoproliferation and interleukin 2 production as indicators of immunotoxicity. / Reid, Lynnda L.; Hastings, Kenneth L.; Gandolfi, A Jay; Ert, Mark Van.

In: Drug and Chemical Toxicology, Vol. 17, No. 1, 1994, p. 1-14.

Research output: Contribution to journalArticle

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