Using bacteria to express and display anti-Plasmodium molecules in the mosquito midgut

Michael A Riehle, Cristina K. Moreira, David Lampe, Carol Lauzon, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Bacteria capable of colonizing mosquito midguts are attractive vehicles for delivering anti-malaria molecules. We genetically engineered Escherichia coli to display two anti-Plasmodium effector molecules, SM1 and phospholipase-A(2), on their outer membrane. Both molecules significantly inhibited Plasmodium berghei development when engineered bacteria were fed to mosquitoes 24 h prior to an infective bloodmeal (SM1 = 41%, PLA2 = 23%). Furthermore, prevalence and numbers of engineered bacteria increased dramatically following a bloodmeal. However, E. coli survived poorly in mosquitoes. Therefore, Enterobacter agglomerans was isolated from mosquitoes and selected for midgut survival by multiple passages through mosquitoes. After four passages, E. agglomerans survivorship increased from 2 days to 2 weeks. Since E. agglomerans is non-pathogenic and widespread, it is an excellent candidate for paratransgenic control strategies.

Original languageEnglish (US)
Pages (from-to)595-603
Number of pages9
JournalInternational Journal for Parasitology
Volume37
Issue number6
DOIs
StatePublished - May 2007
Externally publishedYes

Fingerprint

Plasmodium
Culicidae
Bacteria
Escherichia coli
Plasmodium berghei
Enterobacter
Phospholipases A
Malaria
Survival Rate
Membranes

Keywords

  • Malaria
  • Mosquito
  • Paratransgenesis
  • PhospholipaseA(2)
  • SM1

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Using bacteria to express and display anti-Plasmodium molecules in the mosquito midgut. / Riehle, Michael A; Moreira, Cristina K.; Lampe, David; Lauzon, Carol; Jacobs-Lorena, Marcelo.

In: International Journal for Parasitology, Vol. 37, No. 6, 05.2007, p. 595-603.

Research output: Contribution to journalArticle

Riehle, Michael A ; Moreira, Cristina K. ; Lampe, David ; Lauzon, Carol ; Jacobs-Lorena, Marcelo. / Using bacteria to express and display anti-Plasmodium molecules in the mosquito midgut. In: International Journal for Parasitology. 2007 ; Vol. 37, No. 6. pp. 595-603.
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