Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells

Jeffrey V. Wong, Guang Yao, Joseph R. Nevins, Lingchong You

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Perturbations from environmental, genetic, and pharmacological sources can generate heterogeneous biological responses, even in genetically identical cells. Although these differences have important consequences on cell physiology and survival, they are often subsumed in measurements that average over the population. Here, we describe in detail how variability in adenoviral-mediated gene expression provides an effective means to map dose responses of signaling pathways. Cellcell variability is inherent in gene delivery methods used in cell biology, which makes this approach adaptable to many existing experimental systems. We also discuss strategies to quantify biologically relevant inputs and outputs.

Original languageEnglish (US)
Pages (from-to)221-237
Number of pages17
JournalMethods in Enzymology
Volume497
DOIs
StatePublished - 2011

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Cytology
Cell Physiological Phenomena
Physiology
Adenoviridae
Gene expression
Cell Biology
Cell Survival
Genes
Cells
Pharmacology
Gene Expression
Population

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells. / Wong, Jeffrey V.; Yao, Guang; Nevins, Joseph R.; You, Lingchong.

In: Methods in Enzymology, Vol. 497, 2011, p. 221-237.

Research output: Contribution to journalArticle

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