Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

Meizhong Luo, Yeisoo Yu, Hyeran Kim, Dave Kudrna, Yuichiro Itoh, Robert J. Agate, Esther Melamed, José L. Goicoechea, Jayson Talag, Christopher Mueller, Wenming Wang, Jennifer Currie, Nicholas B. Sisneros, Rod A. Wing, Arthur P. Arnold

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.

Original languageEnglish (US)
Pages (from-to)181-190
Number of pages10
JournalGenomics
Volume87
Issue number1
DOIs
StatePublished - Jan 2006

Keywords

  • Androgen receptor
  • BAC library
  • Bird
  • Genomics
  • Zebra finch

ASJC Scopus subject areas

  • Genetics

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