Utilization of renal slices to evaluate the efficacy of chelating agents for removing mercury from the kidney

R. L. Keith, I. Setiarahardjo, Q. Fernando, H. V. Aposhian, A. J. Gandolfi

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Mercury is an environmental contaminant that preferentially accumulates in the kidney. It has been previously shown using proton-induced X-ray emission analysis that mercury (HgCl2) accumulated in precision-cut rabbit renal cortical slices. In this study, the efficacy of seven chelating agents for the removal of Hg from renal dices has been examined. Rabbits were injected with HgCl2 (10 mg/kg) and 3 h later kidneys were sliced, or renal slices were exposed in vitro to a mildly toxic concentration of HgCl2 (5 x 10-5 M, 4 h). The slices were then treated in vitro with 10 mM concentrations of EDTA, lipoic acid (LA), penicillamine (PA), glutathione (GSH), 1,4-dithiothreitol (DTT), DMSA, or DMPS. DMPS proved to be the most effective in mobilizing Hg from in vivo or in vitro HgCl2-exposed renal tissue (>85% of control after 3 h incubation). Relative efficacies for the seven agents were DMPS > DMSA, PA > DTT, GSH > LA, EDTA. The use of renal slices appears to be a useful in vitro tool for assessing the efficacy of chelating agents on mobilizing accumulated Hg from renal tissue.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalToxicology
Volume116
Issue number1-3
DOIs
StatePublished - Jan 15 1997

Keywords

  • Chelators
  • Emission spectroscopy
  • Kidney slices
  • Metals
  • Proton-induced X-ray emission

ASJC Scopus subject areas

  • Toxicology

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