UV-visible and infrared methods for investigating lipid-rhodopsin membrane interactions

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

We describe experimental UV-visible and Fourier transform infrared (FTIR) spectroscopic methods for characterizing lipid-protein interactions for rhodopsin in a membrane bilayer environment. The combination of FTIR and UV-visible difference spectroscopy is used to monitor the structural and functional changes during rhodopsin activation. Investigations of how membrane lipids stabilize various rhodopsin photoproducts are analogous to mutating the protein in terms of gain or loss of function. Interpretation of the results entails a flexible surface model for explaining membrane lipid-protein interactions through material properties relevant to biological activity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages127-153
Number of pages27
Volume914
ISBN (Print)9781627030229
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume914
ISSN (Print)10643745

Keywords

  • Flexible surface model
  • G protein-coupled receptor
  • Hydrophobic mismatch
  • Infrared spectroscopy
  • Lateral pressure
  • Lipids
  • Membrane curvature
  • Protein-lipid interactions
  • Rhodopsin
  • Vision

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Brown, M. F. (2012). UV-visible and infrared methods for investigating lipid-rhodopsin membrane interactions. In Methods in Molecular Biology (Vol. 914, pp. 127-153). (Methods in Molecular Biology; Vol. 914). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-23-6_8