Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660

Robert E. Johnston, Philip R. Johnson, Mary J. Connell, David C. Montefiori, Ande West, Martha L. Collier, Chad Cecil, Ronald Swanstrom, Jeffrey A Frelinger, Nancy L. Davis

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

VEE replicon particles (VRP), non-propagating vaccine vectors derived from Venezuelan equine encephalitis virus (VEE), were engineered to express immunogens from the cloned isolate SIVsmH-4, combined in a vaccine cocktail and inoculated subcutaneously to immunize rhesus macaques. The virulent, uncloned challenge stock, SIVsmE660, represented a type of heterologous challenge and the intrarectal challenge modeled infection across a mucosal surface. Prechallenge neutralizing antibodies against SIVsmH-4 were induced in all vaccinates, and a prechallenge cellular immune response could be detected in one of six. Post-challenge, virus loads were reduced at the peak, at set point and at termination (41 weeks post-challenge), although these differences did not reach statistical significance. Significantly elevated levels of CD4+ T cells were observed post-challenge. A strong correlation was noted between a net increase in CD4+ T cell count and lowered virus load at set point.

Original languageEnglish (US)
Pages (from-to)4969-4979
Number of pages11
JournalVaccine
Volume23
Issue number42
DOIs
StatePublished - Oct 10 2005
Externally publishedYes

Fingerprint

Venezuelan Equine Encephalitis Viruses
Venezuelan equine encephalitis virus
replicon
Replicon
Macaca
viral load
Virion
Vaccination
Vaccines
T-lymphocytes
vaccination
Viruses
antigens
T-Lymphocytes
vector vaccines
CD4 Lymphocyte Count
Macaca mulatta
Neutralizing Antibodies
neutralizing antibodies
Cellular Immunity

Keywords

  • Replicon
  • SIV
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660. / Johnston, Robert E.; Johnson, Philip R.; Connell, Mary J.; Montefiori, David C.; West, Ande; Collier, Martha L.; Cecil, Chad; Swanstrom, Ronald; Frelinger, Jeffrey A; Davis, Nancy L.

In: Vaccine, Vol. 23, No. 42, 10.10.2005, p. 4969-4979.

Research output: Contribution to journalArticle

Johnston, RE, Johnson, PR, Connell, MJ, Montefiori, DC, West, A, Collier, ML, Cecil, C, Swanstrom, R, Frelinger, JA & Davis, NL 2005, 'Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660', Vaccine, vol. 23, no. 42, pp. 4969-4979. https://doi.org/10.1016/j.vaccine.2005.05.034
Johnston, Robert E. ; Johnson, Philip R. ; Connell, Mary J. ; Montefiori, David C. ; West, Ande ; Collier, Martha L. ; Cecil, Chad ; Swanstrom, Ronald ; Frelinger, Jeffrey A ; Davis, Nancy L. / Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660. In: Vaccine. 2005 ; Vol. 23, No. 42. pp. 4969-4979.
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