Vaccine-induced cellular immune responses differ from innate responses in susceptible and resistant strains of mice infected with Coccidioides posadasii

Lisa Shubitz, Sharon M. Dial, Robert Perrill, Rachael Casement, John N Galgiani

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Susceptibility to Coccidioides spp. varies widely in humans and other mammals and also among individuals within a species. Among strains of mice with various susceptibilities, immunohistopathology revealed that C57BL/6 mice were highly susceptible to the disease following intranasal infection, DBA/2n mice were intermediate, and Swiss-Webster mice were innately resistant. Resistant Swiss-Webster mice developed prominent perivascular/peribronchiolar lymphocytic cuffing and well-formed granulomas with few fungal elements and debris in the necrotic center, surrounded by a mantle of macrophages, lymphocytes, and fibrocytes. Susceptible C57BL/6 mice became moribund between 14 and 18 days postinfection, with overwhelming numbers of neutrophils and spherules and very few T cells, the drastic reduction of which was associated with failure and death, while intermediate DBA/2n mice controlled the fungal burden but demonstrated progressive lung inflammation with prominent suppuration, and they deteriorated clinically. Vaccinated C57BL/6 mice had an early and robust lymphocyte response, which included significantly higher Mac2+, CD3+, and CD4+ cell scores on day 18 than those of innately resistant SW mice and DBA/2n mice; they also had prominent perivascular/ peribronchiolar lymphocytic infiltrates not present in their unvaccinated counterparts, and they appeared to be resolving lesions by day 56 compared to the other two strains, based on significantly lower disease scores and observably smaller and fewer lesions with few spherules and neutrophils.

Original languageEnglish (US)
Pages (from-to)5553-5564
Number of pages12
JournalInfection and Immunity
Volume76
Issue number12
DOIs
StatePublished - Dec 2008

Fingerprint

Coccidioides
Cellular Immunity
Inbred DBA Mouse
Vaccines
Inbred C57BL Mouse
Neutrophils
Lymphocytes
Suppuration
Granuloma
Mammals
Pneumonia
Macrophages
T-Lymphocytes
Infection

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Vaccine-induced cellular immune responses differ from innate responses in susceptible and resistant strains of mice infected with Coccidioides posadasii. / Shubitz, Lisa; Dial, Sharon M.; Perrill, Robert; Casement, Rachael; Galgiani, John N.

In: Infection and Immunity, Vol. 76, No. 12, 12.2008, p. 5553-5564.

Research output: Contribution to journalArticle

@article{bd0dd13d52514f4b98c1dc4f2d583fa2,
title = "Vaccine-induced cellular immune responses differ from innate responses in susceptible and resistant strains of mice infected with Coccidioides posadasii",
abstract = "Susceptibility to Coccidioides spp. varies widely in humans and other mammals and also among individuals within a species. Among strains of mice with various susceptibilities, immunohistopathology revealed that C57BL/6 mice were highly susceptible to the disease following intranasal infection, DBA/2n mice were intermediate, and Swiss-Webster mice were innately resistant. Resistant Swiss-Webster mice developed prominent perivascular/peribronchiolar lymphocytic cuffing and well-formed granulomas with few fungal elements and debris in the necrotic center, surrounded by a mantle of macrophages, lymphocytes, and fibrocytes. Susceptible C57BL/6 mice became moribund between 14 and 18 days postinfection, with overwhelming numbers of neutrophils and spherules and very few T cells, the drastic reduction of which was associated with failure and death, while intermediate DBA/2n mice controlled the fungal burden but demonstrated progressive lung inflammation with prominent suppuration, and they deteriorated clinically. Vaccinated C57BL/6 mice had an early and robust lymphocyte response, which included significantly higher Mac2+, CD3+, and CD4+ cell scores on day 18 than those of innately resistant SW mice and DBA/2n mice; they also had prominent perivascular/ peribronchiolar lymphocytic infiltrates not present in their unvaccinated counterparts, and they appeared to be resolving lesions by day 56 compared to the other two strains, based on significantly lower disease scores and observably smaller and fewer lesions with few spherules and neutrophils.",
author = "Lisa Shubitz and Dial, {Sharon M.} and Robert Perrill and Rachael Casement and Galgiani, {John N}",
year = "2008",
month = "12",
doi = "10.1128/IAI.00885-08",
language = "English (US)",
volume = "76",
pages = "5553--5564",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Vaccine-induced cellular immune responses differ from innate responses in susceptible and resistant strains of mice infected with Coccidioides posadasii

AU - Shubitz, Lisa

AU - Dial, Sharon M.

AU - Perrill, Robert

AU - Casement, Rachael

AU - Galgiani, John N

PY - 2008/12

Y1 - 2008/12

N2 - Susceptibility to Coccidioides spp. varies widely in humans and other mammals and also among individuals within a species. Among strains of mice with various susceptibilities, immunohistopathology revealed that C57BL/6 mice were highly susceptible to the disease following intranasal infection, DBA/2n mice were intermediate, and Swiss-Webster mice were innately resistant. Resistant Swiss-Webster mice developed prominent perivascular/peribronchiolar lymphocytic cuffing and well-formed granulomas with few fungal elements and debris in the necrotic center, surrounded by a mantle of macrophages, lymphocytes, and fibrocytes. Susceptible C57BL/6 mice became moribund between 14 and 18 days postinfection, with overwhelming numbers of neutrophils and spherules and very few T cells, the drastic reduction of which was associated with failure and death, while intermediate DBA/2n mice controlled the fungal burden but demonstrated progressive lung inflammation with prominent suppuration, and they deteriorated clinically. Vaccinated C57BL/6 mice had an early and robust lymphocyte response, which included significantly higher Mac2+, CD3+, and CD4+ cell scores on day 18 than those of innately resistant SW mice and DBA/2n mice; they also had prominent perivascular/ peribronchiolar lymphocytic infiltrates not present in their unvaccinated counterparts, and they appeared to be resolving lesions by day 56 compared to the other two strains, based on significantly lower disease scores and observably smaller and fewer lesions with few spherules and neutrophils.

AB - Susceptibility to Coccidioides spp. varies widely in humans and other mammals and also among individuals within a species. Among strains of mice with various susceptibilities, immunohistopathology revealed that C57BL/6 mice were highly susceptible to the disease following intranasal infection, DBA/2n mice were intermediate, and Swiss-Webster mice were innately resistant. Resistant Swiss-Webster mice developed prominent perivascular/peribronchiolar lymphocytic cuffing and well-formed granulomas with few fungal elements and debris in the necrotic center, surrounded by a mantle of macrophages, lymphocytes, and fibrocytes. Susceptible C57BL/6 mice became moribund between 14 and 18 days postinfection, with overwhelming numbers of neutrophils and spherules and very few T cells, the drastic reduction of which was associated with failure and death, while intermediate DBA/2n mice controlled the fungal burden but demonstrated progressive lung inflammation with prominent suppuration, and they deteriorated clinically. Vaccinated C57BL/6 mice had an early and robust lymphocyte response, which included significantly higher Mac2+, CD3+, and CD4+ cell scores on day 18 than those of innately resistant SW mice and DBA/2n mice; they also had prominent perivascular/ peribronchiolar lymphocytic infiltrates not present in their unvaccinated counterparts, and they appeared to be resolving lesions by day 56 compared to the other two strains, based on significantly lower disease scores and observably smaller and fewer lesions with few spherules and neutrophils.

UR - http://www.scopus.com/inward/record.url?scp=57349156574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57349156574&partnerID=8YFLogxK

U2 - 10.1128/IAI.00885-08

DO - 10.1128/IAI.00885-08

M3 - Article

VL - 76

SP - 5553

EP - 5564

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 12

ER -