Abstract
To understand the origin of vacuolar H+-ATPases (V-ATPases) and their cellular functions, the subcellular location of V-H+-ATPases was examined immunologically in root cells of oat seedlings. A V-ATPase complex from oat roots consists of a large peripheral sector (V1) that includes the 70-kD (A) catalytic and the 60-kD (B) regulatory subunits. The soluble V1 complex, thought to be synthesized in the cytoplasm, is assembled with the membrane integral sector (VO) at a yet undefined location. In mature cells, V-ATPase subunits A and B, detected in immunoblots with monoclonal antibodies (Mab) (7A5 and 2E7), were associated mainly with vacuolar membranes (20-22% sucrose) fractionated with an isopycnic sucrose gradient. However, in immature root tip cells, which lack large vacuoles, most of the V-ATPase was localized with the endoplasmic reticulum (ER) at 28 to 31% sucrose where a major ER-resident binding protein equilibrated. The peripheral subunits were also associated with membranes at 22% sucrose, at 31 to 34% sucrose (Golgi), and in plasma membranes at 38% sucrose. Immunogold labeling of root tip cells with Mab 2E7 against subunit B showed gold particles decorating the ER as well as numerous small vesicles (0.1-0.3 μm diameter), presumably provacuoles. The immunological detection of the peripheral subunit B on the ER supports a model in which the V1 sector is assembled with the Vo on the ER. These results support the model in which the central vacuolar membrane originates ultimately from the ER. The presence of V-ATPases on several endomembranes indicates that this pump could participate in diverse functional roles.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1313-1324 |
| Number of pages | 12 |
| Journal | Plant Physiology |
| Volume | 106 |
| Issue number | 4 |
| State | Published - Dec 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Plant Science
Cite this
Vacuolar-type H+-ATPases are associated with the endoplasmic reticulum and provacuoles of root tip cells. / Herman, Eliot M; Li, Xuhang; Su, Robert T.; Larsen, Paul; Hsu, Hei Ti; Sze, Heven.
In: Plant Physiology, Vol. 106, No. 4, 12.1994, p. 1313-1324.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vacuolar-type H+-ATPases are associated with the endoplasmic reticulum and provacuoles of root tip cells
AU - Herman, Eliot M
AU - Li, Xuhang
AU - Su, Robert T.
AU - Larsen, Paul
AU - Hsu, Hei Ti
AU - Sze, Heven
PY - 1994/12
Y1 - 1994/12
N2 - To understand the origin of vacuolar H+-ATPases (V-ATPases) and their cellular functions, the subcellular location of V-H+-ATPases was examined immunologically in root cells of oat seedlings. A V-ATPase complex from oat roots consists of a large peripheral sector (V1) that includes the 70-kD (A) catalytic and the 60-kD (B) regulatory subunits. The soluble V1 complex, thought to be synthesized in the cytoplasm, is assembled with the membrane integral sector (VO) at a yet undefined location. In mature cells, V-ATPase subunits A and B, detected in immunoblots with monoclonal antibodies (Mab) (7A5 and 2E7), were associated mainly with vacuolar membranes (20-22% sucrose) fractionated with an isopycnic sucrose gradient. However, in immature root tip cells, which lack large vacuoles, most of the V-ATPase was localized with the endoplasmic reticulum (ER) at 28 to 31% sucrose where a major ER-resident binding protein equilibrated. The peripheral subunits were also associated with membranes at 22% sucrose, at 31 to 34% sucrose (Golgi), and in plasma membranes at 38% sucrose. Immunogold labeling of root tip cells with Mab 2E7 against subunit B showed gold particles decorating the ER as well as numerous small vesicles (0.1-0.3 μm diameter), presumably provacuoles. The immunological detection of the peripheral subunit B on the ER supports a model in which the V1 sector is assembled with the Vo on the ER. These results support the model in which the central vacuolar membrane originates ultimately from the ER. The presence of V-ATPases on several endomembranes indicates that this pump could participate in diverse functional roles.
AB - To understand the origin of vacuolar H+-ATPases (V-ATPases) and their cellular functions, the subcellular location of V-H+-ATPases was examined immunologically in root cells of oat seedlings. A V-ATPase complex from oat roots consists of a large peripheral sector (V1) that includes the 70-kD (A) catalytic and the 60-kD (B) regulatory subunits. The soluble V1 complex, thought to be synthesized in the cytoplasm, is assembled with the membrane integral sector (VO) at a yet undefined location. In mature cells, V-ATPase subunits A and B, detected in immunoblots with monoclonal antibodies (Mab) (7A5 and 2E7), were associated mainly with vacuolar membranes (20-22% sucrose) fractionated with an isopycnic sucrose gradient. However, in immature root tip cells, which lack large vacuoles, most of the V-ATPase was localized with the endoplasmic reticulum (ER) at 28 to 31% sucrose where a major ER-resident binding protein equilibrated. The peripheral subunits were also associated with membranes at 22% sucrose, at 31 to 34% sucrose (Golgi), and in plasma membranes at 38% sucrose. Immunogold labeling of root tip cells with Mab 2E7 against subunit B showed gold particles decorating the ER as well as numerous small vesicles (0.1-0.3 μm diameter), presumably provacuoles. The immunological detection of the peripheral subunit B on the ER supports a model in which the V1 sector is assembled with the Vo on the ER. These results support the model in which the central vacuolar membrane originates ultimately from the ER. The presence of V-ATPases on several endomembranes indicates that this pump could participate in diverse functional roles.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028259120&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0028259120
VL - 106
SP - 1313
EP - 1324
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 4
ER -