Vacuole accumulation of storage protein and lectin expressed in transgenic tobacco seeds

Eliot M Herman, Maarten J. Chrispeels, Leslie M. Hoffman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The expression of proteins in transgenic plants offers an elegant means to examine targeting signals used for transport to intracellular sites of accumulation. We have used electron microscopic immunogold procedures to localize several different storage proteins and lectins expressed in transgenic tobacco seeds. The objective of these studies is to characterize targeting signals which permit translocation and accumulation in protein storage vacuoles (protein bodies). Vacuolar proteins such as phaseolin and phytohemagglutinin (PHA) are correctly transported to the protein storage vacuoles of transgenic tobacco seeds. Site-directed mutagenesis was used to change Asn-linked glycosylation sites of PHA. Minus glycan PHA was accumulated in the protein storage vacuoles indicating that glycans do not confer targeting information. Zein the non-vacuolar storage protein of maize accumulates in the protein storage vacuoles indicating that deposition occurs in some proteins which may lack vacuolar targeting signals.

Original languageEnglish (US)
Pages (from-to)37-45
Number of pages9
JournalCell Biology International Reports
Volume13
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Vacuoles
Lectins
Tobacco
Seeds
Proteins
Phytohemagglutinins
Polysaccharides
Zein
Genetically Modified Plants
Site-Directed Mutagenesis
Glycosylation
Zea mays
Electrons

ASJC Scopus subject areas

  • Cell Biology

Cite this

Vacuole accumulation of storage protein and lectin expressed in transgenic tobacco seeds. / Herman, Eliot M; Chrispeels, Maarten J.; Hoffman, Leslie M.

In: Cell Biology International Reports, Vol. 13, No. 1, 1989, p. 37-45.

Research output: Contribution to journalArticle

@article{ae45eb105f2d4174a8fc20b7e0143992,
title = "Vacuole accumulation of storage protein and lectin expressed in transgenic tobacco seeds",
abstract = "The expression of proteins in transgenic plants offers an elegant means to examine targeting signals used for transport to intracellular sites of accumulation. We have used electron microscopic immunogold procedures to localize several different storage proteins and lectins expressed in transgenic tobacco seeds. The objective of these studies is to characterize targeting signals which permit translocation and accumulation in protein storage vacuoles (protein bodies). Vacuolar proteins such as phaseolin and phytohemagglutinin (PHA) are correctly transported to the protein storage vacuoles of transgenic tobacco seeds. Site-directed mutagenesis was used to change Asn-linked glycosylation sites of PHA. Minus glycan PHA was accumulated in the protein storage vacuoles indicating that glycans do not confer targeting information. Zein the non-vacuolar storage protein of maize accumulates in the protein storage vacuoles indicating that deposition occurs in some proteins which may lack vacuolar targeting signals.",
author = "Herman, {Eliot M} and Chrispeels, {Maarten J.} and Hoffman, {Leslie M.}",
year = "1989",
doi = "10.1016/S0309-1651(89)80006-2",
language = "English (US)",
volume = "13",
pages = "37--45",
journal = "Cell Biology International",
issn = "1065-6995",
publisher = "Portland Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Vacuole accumulation of storage protein and lectin expressed in transgenic tobacco seeds

AU - Herman, Eliot M

AU - Chrispeels, Maarten J.

AU - Hoffman, Leslie M.

PY - 1989

Y1 - 1989

N2 - The expression of proteins in transgenic plants offers an elegant means to examine targeting signals used for transport to intracellular sites of accumulation. We have used electron microscopic immunogold procedures to localize several different storage proteins and lectins expressed in transgenic tobacco seeds. The objective of these studies is to characterize targeting signals which permit translocation and accumulation in protein storage vacuoles (protein bodies). Vacuolar proteins such as phaseolin and phytohemagglutinin (PHA) are correctly transported to the protein storage vacuoles of transgenic tobacco seeds. Site-directed mutagenesis was used to change Asn-linked glycosylation sites of PHA. Minus glycan PHA was accumulated in the protein storage vacuoles indicating that glycans do not confer targeting information. Zein the non-vacuolar storage protein of maize accumulates in the protein storage vacuoles indicating that deposition occurs in some proteins which may lack vacuolar targeting signals.

AB - The expression of proteins in transgenic plants offers an elegant means to examine targeting signals used for transport to intracellular sites of accumulation. We have used electron microscopic immunogold procedures to localize several different storage proteins and lectins expressed in transgenic tobacco seeds. The objective of these studies is to characterize targeting signals which permit translocation and accumulation in protein storage vacuoles (protein bodies). Vacuolar proteins such as phaseolin and phytohemagglutinin (PHA) are correctly transported to the protein storage vacuoles of transgenic tobacco seeds. Site-directed mutagenesis was used to change Asn-linked glycosylation sites of PHA. Minus glycan PHA was accumulated in the protein storage vacuoles indicating that glycans do not confer targeting information. Zein the non-vacuolar storage protein of maize accumulates in the protein storage vacuoles indicating that deposition occurs in some proteins which may lack vacuolar targeting signals.

UR - http://www.scopus.com/inward/record.url?scp=2242442873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2242442873&partnerID=8YFLogxK

U2 - 10.1016/S0309-1651(89)80006-2

DO - 10.1016/S0309-1651(89)80006-2

M3 - Article

VL - 13

SP - 37

EP - 45

JO - Cell Biology International

JF - Cell Biology International

SN - 1065-6995

IS - 1

ER -