Validation of a novel method to interrupt sleep in the mouse

Christopher M. Sinton, Della Kovakkattu, Randall S Friese

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Interrupted sleep, fragmented sleep or restricted sleep is a corollary of many psychiatric, neurological and respiratory disorders and also results from disruptive environments such as that of the intensive care unit (ICU). Recent rodent studies have revealed that sleep interruption (SI) can have more significant consequences for cognitive and neurophysiological variables than were expected and may even be equivalent to those of total sleep deprivation. Results from this research are therefore being increasingly recognized for their implications, which may include delayed recovery from critical illness in the ICU. Here we describe in detail a method for interrupting sleep in a murine model, which we had previously adopted to show an increase in mortality after septic insult. Interrupting sleep for 30 s every 2 min over 48 h significantly decreased rapid eye movement (REM) and non-rapid eye movement (NREM) sleep. The technique, which is based on using a standard laboratory orbital shaker to oscillate the cage containing the mouse, can easily be adapted to use different parameters for SI. During recovery, mice exhibited a rebound in REM sleep time and an increase in the depth of NREM sleep as measured by delta (1-4 Hz) power in the electroencephalogram. The changes in sleep both during and after SI showed some differences from those previously observed in the rat using the same SI parameters. In conclusion, the mouse may provide a useful alternative model for studying the effects of SI.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalJournal of Neuroscience Methods
Volume184
Issue number1
DOIs
StatePublished - Oct 30 2009

Fingerprint

Sleep
REM Sleep
Eye Movements
Intensive Care Units
Sleep Deprivation
Nervous System Diseases
Critical Illness
Psychiatry
Electroencephalography
Rodentia
Mortality

Keywords

  • C57Bl/6
  • Intensive care
  • Recovery sleep
  • Sleep episode duration
  • Sleep fragmentation
  • Sleep homeostasis
  • Sleep interruption

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Validation of a novel method to interrupt sleep in the mouse. / Sinton, Christopher M.; Kovakkattu, Della; Friese, Randall S.

In: Journal of Neuroscience Methods, Vol. 184, No. 1, 30.10.2009, p. 71-78.

Research output: Contribution to journalArticle

Sinton, Christopher M. ; Kovakkattu, Della ; Friese, Randall S. / Validation of a novel method to interrupt sleep in the mouse. In: Journal of Neuroscience Methods. 2009 ; Vol. 184, No. 1. pp. 71-78.
@article{d192c8e251a4417bb6af7aa57756b566,
title = "Validation of a novel method to interrupt sleep in the mouse",
abstract = "Interrupted sleep, fragmented sleep or restricted sleep is a corollary of many psychiatric, neurological and respiratory disorders and also results from disruptive environments such as that of the intensive care unit (ICU). Recent rodent studies have revealed that sleep interruption (SI) can have more significant consequences for cognitive and neurophysiological variables than were expected and may even be equivalent to those of total sleep deprivation. Results from this research are therefore being increasingly recognized for their implications, which may include delayed recovery from critical illness in the ICU. Here we describe in detail a method for interrupting sleep in a murine model, which we had previously adopted to show an increase in mortality after septic insult. Interrupting sleep for 30 s every 2 min over 48 h significantly decreased rapid eye movement (REM) and non-rapid eye movement (NREM) sleep. The technique, which is based on using a standard laboratory orbital shaker to oscillate the cage containing the mouse, can easily be adapted to use different parameters for SI. During recovery, mice exhibited a rebound in REM sleep time and an increase in the depth of NREM sleep as measured by delta (1-4 Hz) power in the electroencephalogram. The changes in sleep both during and after SI showed some differences from those previously observed in the rat using the same SI parameters. In conclusion, the mouse may provide a useful alternative model for studying the effects of SI.",
keywords = "C57Bl/6, Intensive care, Recovery sleep, Sleep episode duration, Sleep fragmentation, Sleep homeostasis, Sleep interruption",
author = "Sinton, {Christopher M.} and Della Kovakkattu and Friese, {Randall S}",
year = "2009",
month = "10",
day = "30",
doi = "10.1016/j.jneumeth.2009.07.026",
language = "English (US)",
volume = "184",
pages = "71--78",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Validation of a novel method to interrupt sleep in the mouse

AU - Sinton, Christopher M.

AU - Kovakkattu, Della

AU - Friese, Randall S

PY - 2009/10/30

Y1 - 2009/10/30

N2 - Interrupted sleep, fragmented sleep or restricted sleep is a corollary of many psychiatric, neurological and respiratory disorders and also results from disruptive environments such as that of the intensive care unit (ICU). Recent rodent studies have revealed that sleep interruption (SI) can have more significant consequences for cognitive and neurophysiological variables than were expected and may even be equivalent to those of total sleep deprivation. Results from this research are therefore being increasingly recognized for their implications, which may include delayed recovery from critical illness in the ICU. Here we describe in detail a method for interrupting sleep in a murine model, which we had previously adopted to show an increase in mortality after septic insult. Interrupting sleep for 30 s every 2 min over 48 h significantly decreased rapid eye movement (REM) and non-rapid eye movement (NREM) sleep. The technique, which is based on using a standard laboratory orbital shaker to oscillate the cage containing the mouse, can easily be adapted to use different parameters for SI. During recovery, mice exhibited a rebound in REM sleep time and an increase in the depth of NREM sleep as measured by delta (1-4 Hz) power in the electroencephalogram. The changes in sleep both during and after SI showed some differences from those previously observed in the rat using the same SI parameters. In conclusion, the mouse may provide a useful alternative model for studying the effects of SI.

AB - Interrupted sleep, fragmented sleep or restricted sleep is a corollary of many psychiatric, neurological and respiratory disorders and also results from disruptive environments such as that of the intensive care unit (ICU). Recent rodent studies have revealed that sleep interruption (SI) can have more significant consequences for cognitive and neurophysiological variables than were expected and may even be equivalent to those of total sleep deprivation. Results from this research are therefore being increasingly recognized for their implications, which may include delayed recovery from critical illness in the ICU. Here we describe in detail a method for interrupting sleep in a murine model, which we had previously adopted to show an increase in mortality after septic insult. Interrupting sleep for 30 s every 2 min over 48 h significantly decreased rapid eye movement (REM) and non-rapid eye movement (NREM) sleep. The technique, which is based on using a standard laboratory orbital shaker to oscillate the cage containing the mouse, can easily be adapted to use different parameters for SI. During recovery, mice exhibited a rebound in REM sleep time and an increase in the depth of NREM sleep as measured by delta (1-4 Hz) power in the electroencephalogram. The changes in sleep both during and after SI showed some differences from those previously observed in the rat using the same SI parameters. In conclusion, the mouse may provide a useful alternative model for studying the effects of SI.

KW - C57Bl/6

KW - Intensive care

KW - Recovery sleep

KW - Sleep episode duration

KW - Sleep fragmentation

KW - Sleep homeostasis

KW - Sleep interruption

UR - http://www.scopus.com/inward/record.url?scp=70349255516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349255516&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2009.07.026

DO - 10.1016/j.jneumeth.2009.07.026

M3 - Article

C2 - 19646474

AN - SCOPUS:70349255516

VL - 184

SP - 71

EP - 78

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1

ER -