Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium

Michael W. Harman, Alex E. Hamby, Ross Boltyanskiy, Alexia A. Belperron, Linda K. Bockenstedt, Holger Kress, Eric R. Dufresne, Charles William Wolgemuth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Borrelia burgdorferi, the spirochete that causes Lyme disease, is a tick-transmitted pathogen that requires motility to invade and colonize mammalian and tick hosts. These bacteria use a unique undulating flat-wave shape to penetrate and propel themselves through host tissues. Previous mathematical modeling has suggested that the morphology and motility of these spirochetes depends crucially on the flagellar/cell wall stiffness ratio. Here, we test this prediction using the antibiotic vancomycin to weaken the cell wall. We found that low to moderate doses of vancomycin (≤2.0 μg/mL for 24 h) produced small alterations in cell shape and that as the dose was increased, cell speed decreased. Vancomycin concentrations >1.0 μg/mL also inhibited cell growth and led to bleb formation on a fraction of the cells. To quantitatively assess how vancomycin affects cell stiffness, we used optical traps to bend unflagellated mutants of B. burgdorferi. We found that in the presence of vancomycin, cell wall stiffness gradually decreased over time, with a 40% reduction in the bending stiffness after 36 h. Under the same conditions, the swimming speed of wild-type B. burgdorferi slowed by ∼15%, with only marginal changes to cell morphology. Interestingly, our biophysical model for the swimming dynamics of B. burgdorferi suggested that cell speed should increase with decreasing cell stiffness. We show that this discrepancy can be resolved if the periplasmic volume decreases as the cell wall becomes softer. These results provide a testable hypothesis for how alterations of cell wall stiffness affect periplasmic volume regulation. Furthermore, since motility is crucial to the virulence of B. burgdorferi, the results suggest that sublethal doses of antibiotics could negatively impact spirochete survival by impeding their swim speed, thereby enabling their capture and elimination by phagocytes.

Original languageEnglish (US)
Pages (from-to)746-754
Number of pages9
JournalBiophysical Journal
Volume112
Issue number4
DOIs
StatePublished - Feb 28 2017

Fingerprint

Lyme Disease
Vancomycin
Borrelia burgdorferi
Cell Wall
Bacteria
Spirochaetales
Ticks
Optical Tweezers
Anti-Bacterial Agents
Cell Shape
Blister
Phagocytes
Virulence
Growth

ASJC Scopus subject areas

  • Biophysics

Cite this

Harman, M. W., Hamby, A. E., Boltyanskiy, R., Belperron, A. A., Bockenstedt, L. K., Kress, H., ... Wolgemuth, C. W. (2017). Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium. Biophysical Journal, 112(4), 746-754. https://doi.org/10.1016/j.bpj.2016.12.039

Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium. / Harman, Michael W.; Hamby, Alex E.; Boltyanskiy, Ross; Belperron, Alexia A.; Bockenstedt, Linda K.; Kress, Holger; Dufresne, Eric R.; Wolgemuth, Charles William.

In: Biophysical Journal, Vol. 112, No. 4, 28.02.2017, p. 746-754.

Research output: Contribution to journalArticle

Harman, MW, Hamby, AE, Boltyanskiy, R, Belperron, AA, Bockenstedt, LK, Kress, H, Dufresne, ER & Wolgemuth, CW 2017, 'Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium', Biophysical Journal, vol. 112, no. 4, pp. 746-754. https://doi.org/10.1016/j.bpj.2016.12.039
Harman MW, Hamby AE, Boltyanskiy R, Belperron AA, Bockenstedt LK, Kress H et al. Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium. Biophysical Journal. 2017 Feb 28;112(4):746-754. https://doi.org/10.1016/j.bpj.2016.12.039
Harman, Michael W. ; Hamby, Alex E. ; Boltyanskiy, Ross ; Belperron, Alexia A. ; Bockenstedt, Linda K. ; Kress, Holger ; Dufresne, Eric R. ; Wolgemuth, Charles William. / Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium. In: Biophysical Journal. 2017 ; Vol. 112, No. 4. pp. 746-754.
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