Variably lytic infection dynamics of large Bacteroidetes podovirus phi38: 1 against two Cellulophaga baltica host strains

Vinh T. Dang, Cristina Howard-Varona, Sarah Schwenck, Matthew Sullivan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Bacterial viruses (phages) influence global biogeochemical cycles by modulating bacterial mortality, metabolic output and evolution. However, our understanding of phage infections is limited by few methods and environmentally relevant model systems. Prior work showed that Cellulophaga baltica phage φ{symbol}38:1 infects its original host lytically, and an alternative host either delayed lytically or lysogenically. Here we investigate these infections through traditional and marker-based approaches, and introduce geneELISA for high-throughput examination of phage-host interactions. All methods confirmed the lytic, original host infection (70-80min latent period;approximately eight phages produced per cell), but alternative host assays were more challenging. A 4.5h experiment detected no phage production by plaque assay, whereas phageFISH and geneELISA revealed phage genome replication and a latent period ≥150min. Longer experiments (26h) suggested an 11h latent period and a burst size of 871 by plaque assay, whereas phageFISH identified cell lysis starting at

Original languageEnglish (US)
Pages (from-to)4659-4671
Number of pages13
JournalEnvironmental Microbiology
Volume17
Issue number11
DOIs
StatePublished - Nov 1 2015

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Cellulophaga baltica
Bacteroidetes
host strains
Baltica
bacteriophages
Bacteriophages
Infection
infection
latent period
assay
alternative hosts
biogeochemical cycle
lysis
assays
virus
genome
experiment
mortality
biogeochemical cycles
Genome

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Variably lytic infection dynamics of large Bacteroidetes podovirus phi38 : 1 against two Cellulophaga baltica host strains. / Dang, Vinh T.; Howard-Varona, Cristina; Schwenck, Sarah; Sullivan, Matthew.

In: Environmental Microbiology, Vol. 17, No. 11, 01.11.2015, p. 4659-4671.

Research output: Contribution to journalArticle

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