Variation of surface charge density in monoclonal bacterial populations

Implications for transport through porous media

James C Baygents, J. R. Glynn, O. Albinger, B. K. Biesemeyer, Kimberly L Ogden, Robert G Arnold

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The forced convection of a monodisperse, monoclonal suspension of bacteria through a uniform, saturated porous medium has been investigated. Bench-scale column studies were carried out to measure the removal of microorganisms from suspension due to attachment to the surfaces of the solid phase. The columns were packed with 40-μm borosilicate glass beads, and bacterial sorption was measured as a function of depth in the column using a leucine radiolabel assay. The strains A1264 and CD1 were examined separately. Colloid filtration theory was used to interpret the data, and the average, or effective, affinity of the bacteria for the glass beads was found to decrease with distance traveled through the column. It is postulated that, under these circumstances, the cell/collector affinity (that is, the collision efficiency α) varied due to intrapopulational differences in bacterial surface characteristics. A simple bimodal probability density function, consisting of two Dirac delta functions, was found to satisfactorily represent the α distribution in the original bacterial population. This form of the distribution function was supported by capillary electrophoresis measurements on the bacteria, which showed intrapopulational differences in the surface charge density under the conditions of the transport experiments. These variations in surface charge density are significant in as much as they give rise to substantial differences in the colloidal interaction potentials and, presumably, large differences in cell affinity for negatively charged collectors such as glass beads or quartz.

Original languageEnglish (US)
Pages (from-to)1596-1603
Number of pages8
JournalEnvironmental Science and Technology
Volume32
Issue number11
DOIs
StatePublished - Jun 1 1998

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Surface charge
Charge density
Porous materials
porous medium
Bacteria
glass
Suspensions
bacterium
Delta functions
Glass
Capillary electrophoresis
Quartz
Borosilicate glass
Forced convection
Colloids
Leucine
Microorganisms
Probability density function
Distribution functions
Sorption

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Environmental Engineering

Cite this

Variation of surface charge density in monoclonal bacterial populations : Implications for transport through porous media. / Baygents, James C; Glynn, J. R.; Albinger, O.; Biesemeyer, B. K.; Ogden, Kimberly L; Arnold, Robert G.

In: Environmental Science and Technology, Vol. 32, No. 11, 01.06.1998, p. 1596-1603.

Research output: Contribution to journalArticle

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