Variations in the expressed antimicrobial peptide repertoire of northern leopard frog (Rana pipiens) populations suggest intraspecies differences in resistance to pathogens

Jacob A. Tennessen, Douglas C. Woodhams, Pierre Chaurand, Laura K. Reinert, David D Billheimer, Yu Shyr, Richard M. Caprioli, Michael S. Blouin, Louise A. Rollins-Smith

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The northern leopard frog (Rana pipiens or Lithobates pipiens) is historically found in most of the provinces of Canada and the northern and southwest states of the United States. In the last 50 years, populations have suffered significant losses, especially in the western regions of the species range. Using a peptidomics approach, we show that the pattern of expressed antimicrobial skin peptides of frogs from three geographically separated populations are distinct, and we report the presence of four peptides (brevinin-1Pg, brevinin-1Pl, ranatuerin-2Pb, and ranatuerin-2Pc) that have not previously been found in skin secretions. The differences in expressed peptides reflect differences in the distribution of alleles for the newly described Brevinin1.1 locus in the three populations. When enriched peptide mixtures were tested for their ability to inhibit growth of the pathogenic amphibian chytrid (Batrachochytrium dendrobatidis), peptides from Minnesota or Vermont frogs were more effective that peptides from Michigan frogs. Four of the purified peptides were tested for their ability to inhibit growth of two bacterial pathogens (Aeromonas hydrophila and Staphylococcus epidermidis) and B. dendrobatidis. Three of the four were effective inhibitors of B. dendrobatidis and S. epidermidis, but none inhibited A. hydrophila. We interpret these differences in expression and activity of antimicrobial peptides as evidence to suggest that each population may have been selected to express a suite of peptides that reflects current and past encounters with skin microbes.

Original languageEnglish (US)
Pages (from-to)1247-1257
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume33
Issue number12
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

Rana pipiens
Peptides
Chytridiomycota
Population
Anura
Aeromonas hydrophila
Staphylococcus epidermidis
Skin
Amphibians
Growth
Canada
Alleles

Keywords

  • Aeromonas hydrophila
  • Antimicrobial peptides
  • Batrachochytrium dendrobatidis
  • Brevinin-1
  • Lithobates
  • Mass spectrometry
  • Peptidomics
  • Rana palustris
  • Rana pipiens
  • Ranatuerin-2
  • Staphylococcus epidermidis
  • Temporin

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Variations in the expressed antimicrobial peptide repertoire of northern leopard frog (Rana pipiens) populations suggest intraspecies differences in resistance to pathogens. / Tennessen, Jacob A.; Woodhams, Douglas C.; Chaurand, Pierre; Reinert, Laura K.; Billheimer, David D; Shyr, Yu; Caprioli, Richard M.; Blouin, Michael S.; Rollins-Smith, Louise A.

In: Developmental and Comparative Immunology, Vol. 33, No. 12, 12.2009, p. 1247-1257.

Research output: Contribution to journalArticle

Tennessen, Jacob A. ; Woodhams, Douglas C. ; Chaurand, Pierre ; Reinert, Laura K. ; Billheimer, David D ; Shyr, Yu ; Caprioli, Richard M. ; Blouin, Michael S. ; Rollins-Smith, Louise A. / Variations in the expressed antimicrobial peptide repertoire of northern leopard frog (Rana pipiens) populations suggest intraspecies differences in resistance to pathogens. In: Developmental and Comparative Immunology. 2009 ; Vol. 33, No. 12. pp. 1247-1257.
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abstract = "The northern leopard frog (Rana pipiens or Lithobates pipiens) is historically found in most of the provinces of Canada and the northern and southwest states of the United States. In the last 50 years, populations have suffered significant losses, especially in the western regions of the species range. Using a peptidomics approach, we show that the pattern of expressed antimicrobial skin peptides of frogs from three geographically separated populations are distinct, and we report the presence of four peptides (brevinin-1Pg, brevinin-1Pl, ranatuerin-2Pb, and ranatuerin-2Pc) that have not previously been found in skin secretions. The differences in expressed peptides reflect differences in the distribution of alleles for the newly described Brevinin1.1 locus in the three populations. When enriched peptide mixtures were tested for their ability to inhibit growth of the pathogenic amphibian chytrid (Batrachochytrium dendrobatidis), peptides from Minnesota or Vermont frogs were more effective that peptides from Michigan frogs. Four of the purified peptides were tested for their ability to inhibit growth of two bacterial pathogens (Aeromonas hydrophila and Staphylococcus epidermidis) and B. dendrobatidis. Three of the four were effective inhibitors of B. dendrobatidis and S. epidermidis, but none inhibited A. hydrophila. We interpret these differences in expression and activity of antimicrobial peptides as evidence to suggest that each population may have been selected to express a suite of peptides that reflects current and past encounters with skin microbes.",
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AU - Reinert, Laura K.

AU - Billheimer, David D

AU - Shyr, Yu

AU - Caprioli, Richard M.

AU - Blouin, Michael S.

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