Varicella zoster-specific CD4+Foxp3+ T cells accumulate after cutaneous antigen challenge in humans

Milica Vukmanovic-Stejic, Daisy Sandhu, Toni O. Sobande, Elaine Agius, Katie E. Lacy, Natalie Riddell, Sandra Montez, One B. Dintwe, Thomas J. Scriba, Judith Breuer, Janko Nikolich-Zugich, Graham Ogg, Malcolm H.A. Rustin, Arne N. Akbar

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

We investigated the relationship between varicella zoster virus (VZV)-specific memory CD4+ T cells and CD4+Foxp3+ regulatory T cells (Tregs) that accumulate after intradermal challenge with a VZV skin test Ag. VZV-specific CD4+ T cells were identified with a MHC class II tetramer or by intracellular staining for either IFN-g or IL-2 after Ag rechallenge in vitro. VZV-specific T cells, mainly of a central memory (CD45RA2CD27+) phenotype, accumulate at the site of skin challenge compared with the blood of the same individuals. This resulted in part from local proliferation because >50% of tetramer defined Ag-specific CD4+ T cells in the skin expressed the cell cycle marker Ki67. CD4+Foxp3+ T cells had the characteristic phenotype of Tregs, namely CD25hiCD127loCD39hi in both unchallenged and VZV challenged skin and did not secrete IFN-g or IL-2 after antigenic restimulation. The CD4+Foxp3+ T cells from unchallenged skin had suppressive activity, because their removal led to an increase in cytokine secretion after activation. After VZVAg injection, Foxp3+CD25hiCD127loCD39hi T cells were also found within the VZV tetramer population. Their suppressive activity could not be directly assessed by CD25 depletion because activated T cells in the skin were also CD25+. Nevertheless, there was an inverse correlation between decreased VZV skin responses and proportion of CD4+Foxp 3+ T cells present, indicating indirectly their inhibitory activity in vivo. These results suggest a linkage between the expansion of Ag-specific CD4+ T cells and CD4+ Tregs that may provide controlled responsiveness during Ag-specific stimulation in tissues.

Original languageEnglish (US)
Pages (from-to)977-986
Number of pages10
JournalJournal of Immunology
Volume190
Issue number3
DOIs
StatePublished - Feb 1 2013

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Varicella zoster-specific CD<sub>4</sub>+Foxp<sub>3</sub>+ T cells accumulate after cutaneous antigen challenge in humans'. Together they form a unique fingerprint.

  • Cite this

    Vukmanovic-Stejic, M., Sandhu, D., Sobande, T. O., Agius, E., Lacy, K. E., Riddell, N., Montez, S., Dintwe, O. B., Scriba, T. J., Breuer, J., Nikolich-Zugich, J., Ogg, G., Rustin, M. H. A., & Akbar, A. N. (2013). Varicella zoster-specific CD4+Foxp3+ T cells accumulate after cutaneous antigen challenge in humans. Journal of Immunology, 190(3), 977-986. https://doi.org/10.4049/jimmunol.120133