Abstract
Alpha tocopheryl succinate (α-TOS) is a non-toxic vitamin E analog under study for its anti-cancer properties. In an earlier study, we showed that α-TOS, when used in combination with non-matured dendritic cells (nmDC) to treat pre-established tumors, acts as an effective adjuvant. In this study, we have used vesiculated α-TOS (Vα-TOS), a more soluble form of α-TOS that is relevant for clinical use, in combination with dendritic cells to treat pre-established murine tumors. We demonstrate that Vα-TOS kills tumor cells in vitro and inhibits the growth of pre-established murine lung carcinoma (3LLD122) as effectively as α-TOS. The combination of Vα-TOS plus non-matured or TNF-α-matured DC is more effective at inhibiting the growth of established tumors than Vα-TOS alone. We also observed that Vα-TOS induces expression of heat shock proteins in tumor cells and that co-incubation of non-matured DC with lysate derived from Vα-TOS-treated tumor cells leads to DC maturation evidenced by up-regulation of co-stimulatory molecules and secretion of IL-12p70. This study therefore demonstrates the immunomodulatory properties of Vα-TOS that may account for its adjuvant effect when combined with DC vaccines to treat established tumors.
Original language | English (US) |
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Pages (from-to) | 166-177 |
Number of pages | 12 |
Journal | Cancer Immunology, Immunotherapy |
Volume | 55 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2006 |
Keywords
- Dendritic cell
- Heat shock proteins
- Immunotherapy
- Vaccine
- α-tocopheryl succinate
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Oncology
- Cancer Research