Viradel method for detection of rota virus from seawater

Sagar M. Goyal, Charles P. Gerba

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

This report describes the use of a virus adsorption-elution (Viradel) technique for the concentration and detection of rotavirus from seawater. Simian rotavirus SA-11 was used as a model virus because it can be readily propagated and plaqued in vitro. The virus adsorbed optimally to fiberglass epoxy filters when seawater was adjusted to pH 3.5 and aluminum chloride added to a concentration of 1.0 mM. Adsorbed virus was eluted with 6% beef extract at pH 10.5 and was further concentrated to a smaller volume by a modification of the organic flocculation method. Using this method, in conjunction with an indirect immunofluorescence test, rotavirus was detected in sewage-contaminated seawater.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalJournal of Virological Methods
Volume7
Issue number5-6
DOIs
StatePublished - Dec 1983

Keywords

  • rotavirus marine pollution concentration method human enteric viruses

ASJC Scopus subject areas

  • Virology

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