Virus control of trafficking from sorting endosomes

Sebastian Zeltzer, Carol A. Zeltzer, Suzu Igarashi, Jean Wilson, Julie G. Donaldson, Felicia Goodrum

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The maintenance of cell surface proteins is critical to the ability of a cell to sense and respond to information in its environment. As such, modulation of cell surface composition and receptor trafficking is a potentially important target of control in virus infection. Sorting endosomes (SEs) are control stations regulating the recycling or degradation of internalized plasma membrane proteins. Here we report that human cytomegalovirus (HCMV), a ubiquitous betaherpesvirus, alters the fate of internalized clathrin-independent endocytosis (CIE) cargo proteins, retaining them in virally reprogrammed SEs. We show that the small G protein ARF6 (ADP ribosylation factor 6), a regulator of CIE trafficking, is highly associated with SE membranes relative to uninfected cells. Combined with the observation of accumulated CIE cargo at the SE, these results suggest that infection diminishes the egress of ARF6 and its cargo from the SE. Expression of ubiquitin-specific protease 6 (USP6), also known as TRE17, was sufficient to restore ARF6 and some ARF6 cargo trafficking to the cell surface in infected cells. The USP activity of TRE17 was required to rescue both ARF6 and associated cargo from SE retention in infection. The finding that TRE17 expression does not rescue the trafficking of all CIE cargos retained at SEs in infection suggests that HCMV hijacks the normal sorting machinery and selectively sorts specific cargos into endocytic microdomains that are subject to alternative sorting fates. IMPORTANCE Cells maintain their surface composition, take up nutrients, and respond to their environment through the internalization and recycling of cargo at the cell surface through endocytic trafficking pathways. During infection with human cytomegalovirus (HCMV), host endocytic membranes are reorganized into a juxtanuclear structure associated with viral assembly and egress. Less appreciated is the effect of this reorganization on the trafficking of host proteins through the endocytic pathway. We show that HCMV retains internalized cargo and the effector of clathrin-independent endocytosis at sorting endosomes. The retention of some cargo, but not all, was reversed by overexpression of a ubiquitin-specific protease, TRE17. Our results demonstrate that HCMV induces profound reprogramming of endocytic trafficking and influences cargo sorting decisions. Further, our work suggests the presence of a novel ubiquitin-regulated checkpoint for the recycling of cargo from sorting endosome. These findings have important implications for host signaling and immune pathways in the context of HCMV infection.

Original languageEnglish (US)
Article numbere00683-18
JournalmBio
Volume9
Issue number4
DOIs
StatePublished - Jul 1 2018

Fingerprint

Endosomes
Clathrin
Viruses
Endocytosis
Cytomegalovirus
Recycling
Ubiquitin-Specific Proteases
Infection
Membrane Proteins
Virus Assembly
Membranes
Monomeric GTP-Binding Proteins
Cytomegalovirus Infections
Cell Surface Receptors
Protein Transport
Virus Diseases
Ubiquitin
Blood Proteins
Maintenance
Cell Membrane

Keywords

  • ARF6
  • Cytomegalovirus
  • Endocytic trafficking
  • Endosomes
  • Herpesviruses
  • TRE17
  • USP6

ASJC Scopus subject areas

  • Microbiology
  • Virology

Cite this

Zeltzer, S., Zeltzer, C. A., Igarashi, S., Wilson, J., Donaldson, J. G., & Goodrum, F. (2018). Virus control of trafficking from sorting endosomes. mBio, 9(4), [e00683-18]. https://doi.org/10.1128/mBio.00683-18

Virus control of trafficking from sorting endosomes. / Zeltzer, Sebastian; Zeltzer, Carol A.; Igarashi, Suzu; Wilson, Jean; Donaldson, Julie G.; Goodrum, Felicia.

In: mBio, Vol. 9, No. 4, e00683-18, 01.07.2018.

Research output: Contribution to journalArticle

Zeltzer, S, Zeltzer, CA, Igarashi, S, Wilson, J, Donaldson, JG & Goodrum, F 2018, 'Virus control of trafficking from sorting endosomes', mBio, vol. 9, no. 4, e00683-18. https://doi.org/10.1128/mBio.00683-18
Zeltzer S, Zeltzer CA, Igarashi S, Wilson J, Donaldson JG, Goodrum F. Virus control of trafficking from sorting endosomes. mBio. 2018 Jul 1;9(4). e00683-18. https://doi.org/10.1128/mBio.00683-18
Zeltzer, Sebastian ; Zeltzer, Carol A. ; Igarashi, Suzu ; Wilson, Jean ; Donaldson, Julie G. ; Goodrum, Felicia. / Virus control of trafficking from sorting endosomes. In: mBio. 2018 ; Vol. 9, No. 4.
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