Virus persistence in groundwater

M. V. Yates, Charles P Gerba, L. M. Kelley

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

More than 50% of the outbreaks of waterborne disease in the United States are due to the consumption of contaminated groundwater. An estimated 65% of the cases in these outbreaks are caused by enteric viruses. Little, however, is known about the persistence of viruses in groundwater. The purpose of this study was to determine whether measurable chemical and physical factors correlate with virus survival in groundwater. Groundwater samples were obtained from 11 sites throughout the United States. Water temperature was measured at the time of collection. Several physical and chemical characteristics, including pH, nitrates, turbidity, and hardness, were determined for each sample. Separate water samples were inoculated with each of three viruses (poliovirus 1, echovirus 1, and MS-2 coliphage) and incubated at the in situ groundwater temperature; selected samples were also incubated at other temperatures. Assays were performed at predetermined intervals over a 30-day period to determine the number of infective viruses remaining. Multiple regression analysis revealed that temperature was the only variable significantly correlated with the decay rates of all three viruses. No significant differences were found among the decay rates of the three viruses, an indication that MS-2 coliphage might be used as a model of animal virus survival in groundwater.

Original languageEnglish (US)
Pages (from-to)778-781
Number of pages4
JournalApplied and Environmental Microbiology
Volume49
Issue number4
StatePublished - 1985

Fingerprint

Groundwater
virus
groundwater
persistence
Viruses
viruses
coliphages
Coliphages
Temperature
Disease Outbreaks
deterioration
waterborne diseases
Enterovirus C
sampling
Enterovirus
temperature
Human Enterovirus B
Poliovirus
Water
waterborne disease

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Yates, M. V., Gerba, C. P., & Kelley, L. M. (1985). Virus persistence in groundwater. Applied and Environmental Microbiology, 49(4), 778-781.

Virus persistence in groundwater. / Yates, M. V.; Gerba, Charles P; Kelley, L. M.

In: Applied and Environmental Microbiology, Vol. 49, No. 4, 1985, p. 778-781.

Research output: Contribution to journalArticle

Yates, MV, Gerba, CP & Kelley, LM 1985, 'Virus persistence in groundwater', Applied and Environmental Microbiology, vol. 49, no. 4, pp. 778-781.
Yates, M. V. ; Gerba, Charles P ; Kelley, L. M. / Virus persistence in groundwater. In: Applied and Environmental Microbiology. 1985 ; Vol. 49, No. 4. pp. 778-781.
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