Virus-specific read-through codon preference affects infectivity of chimeric cucumber green mottle mosaic viruses displaying a dengue virus epitope

Rofina Yasmin Othman, Pak Guan Teoh, Aikseng Ooi, Sazaly Abubakar

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A Cucumber green mottle mosaic virus (CGMMV) was used to present a truncated dengue virus type 2 envelope (E) protein binding region from amino acids 379 to 423 (EB4). The EB4 gene was inserted at the terminal end of the CGMMV coat protein (CP) open reading frame (ORF). Read-through sequences of TMV or CGMMV, CAA-UAG-CAA-UUA, or AAA-UAG-CAA-UUA were, respectively, inserted in between the CP and the EB4 genes. The chimeric clones, pRT, pRG, and pCG+FSRTRE, were transcribed into full-length capped recombinant CGMMV transcripts. Only constructs with the wild-type CGMMV read-through sequence yielded infectious viruses following infection of host plant, muskmelon (Cucumis melo) leaves. The ratio of modified to unmodified CP for the read-through expression clone developed was also found to be approximately 1:1, higher than what has been previously reported. It was also observed that infectivity was not affected by differences in pI between the chimera and its wild counterpart. Analysis of recombinant viruses after 21-days-postinculation (dpi) revealed that deletions occurred resulting in partial reversions of the viral population to near wild type and suggesting that this would be the limiting harvest period for obtaining true to type recombinants with this construct.

Original languageEnglish (US)
Article number781712
JournalJournal of Biomedicine and Biotechnology
Volume2009
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Mosaic Viruses
Cucumis sativus
Dengue Virus
Viruses
Codon
Epitopes
Capsid Proteins
Clone Cells
Cucumis melo
Virus Diseases
Protein Binding
Genes
Open Reading Frames
Amino Acids
Population

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Virus-specific read-through codon preference affects infectivity of chimeric cucumber green mottle mosaic viruses displaying a dengue virus epitope. / Othman, Rofina Yasmin; Teoh, Pak Guan; Ooi, Aikseng; Abubakar, Sazaly.

In: Journal of Biomedicine and Biotechnology, Vol. 2009, 781712, 2009.

Research output: Contribution to journalArticle

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