Visualizing muscle cell migration in situ

Brian Knight, Christina M Laukaitis, Nasreen Akhtar, Neil A. Hotchin, Magnus Edlund, Alan Rick Horwitz

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Background: Cell migration has been studied extensively by manipulating and observing cells bathed in putative chemotactic or chemokinetic agents on planar substrates. This environment differs from that in vivo and, consequently, the cells can behave abnormally. Embryo slices provide an optically accessible system for studying cellular navigation pathways during development. We extended this system to observe the migration of muscle precursors from the somite into the forelimb, their cellular morphology, and the localization of green fluorescent protein (GFP)-tagged adhesion-related molecules under normal and perturbed conditions. Results: Muscle precursors initiated migration synchronously and migrated in broad, rather than highly defined, regions. Bursts of directed migration were followed by periods of meandering or extension and retraction of cell protrusions. Although paxillin did not localize to discernible intracellular structures, we found that α-actinin localized to linear, punctate structures, and the α5 integrin to some focal complexes and/or vesicle-like concentrations. Alterations in the expression of adhesion molecules inhibited migration. The muscle precursors migrating in situ formed unusually large, long-lived protrusions that were polarized in the direction of migration. Unlike wild-type Rac, a constitutively active Rac localized continuously around the cell surface and promoted random protrusive activity and migration. Conclusions: The observation of cellular migration and the dynamics of molecular organization at high temporal and spatial resolution in situ is feasible. Migration from the somite to the wing bud is discontinuous and not highly stereotyped. In situ, local activation of Rac appears to produce large protrusions, which in turn, leads to directed migration. Adhesion can also regulate migration.

Original languageEnglish (US)
Pages (from-to)576-585
Number of pages10
JournalCurrent Biology
Volume10
Issue number10
DOIs
StatePublished - May 1 2000
Externally publishedYes

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cell movement
myocytes
Muscle Cells
Cell Movement
Muscle
Adhesion
Cells
adhesion
Somites
Muscles
muscles
Paxillin
Actinin
Molecules
cells
Green Fluorescent Proteins
Integrins
Forelimb
molecular dynamics
integrins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Knight, B., Laukaitis, C. M., Akhtar, N., Hotchin, N. A., Edlund, M., & Horwitz, A. R. (2000). Visualizing muscle cell migration in situ. Current Biology, 10(10), 576-585. https://doi.org/10.1016/S0960-9822(00)00486-3

Visualizing muscle cell migration in situ. / Knight, Brian; Laukaitis, Christina M; Akhtar, Nasreen; Hotchin, Neil A.; Edlund, Magnus; Horwitz, Alan Rick.

In: Current Biology, Vol. 10, No. 10, 01.05.2000, p. 576-585.

Research output: Contribution to journalArticle

Knight, B, Laukaitis, CM, Akhtar, N, Hotchin, NA, Edlund, M & Horwitz, AR 2000, 'Visualizing muscle cell migration in situ', Current Biology, vol. 10, no. 10, pp. 576-585. https://doi.org/10.1016/S0960-9822(00)00486-3
Knight B, Laukaitis CM, Akhtar N, Hotchin NA, Edlund M, Horwitz AR. Visualizing muscle cell migration in situ. Current Biology. 2000 May 1;10(10):576-585. https://doi.org/10.1016/S0960-9822(00)00486-3
Knight, Brian ; Laukaitis, Christina M ; Akhtar, Nasreen ; Hotchin, Neil A. ; Edlund, Magnus ; Horwitz, Alan Rick. / Visualizing muscle cell migration in situ. In: Current Biology. 2000 ; Vol. 10, No. 10. pp. 576-585.
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