Vitamin D-Dependent Transcriptional Regulation of the Intestinal Plasma Membrane Calcium Pump

Thomas L Pannabecker, J. S. Chandler, R. H. Wasserman

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH) 2D3], was shown to increase intestinal plasma membrane calcium pump (PMCA) gene expression. The present study was done to determine whether gene transcription is involved in this process. Nuclei were isolated from duodena of vitamin-D-deficient chicks given 1,25(OH) 2D3 intracardially at various times before experiment. The abundance of PMCA RNA in the nuclear and total cellular fractions, measured by a ribonuclease protection assay, was significantly increased above control values at 1.5 hr. and maximized at 3 hr. post-dose. As shown, cross-contamination of nuclear PMCA RNA by cytosolic RNA cannot account for these results. These studies are the first to show that 1,25(OH) 2D3 regulates expression of a plasma membrane calcium pump gene by increasing the rate of transcription.

Original languageEnglish (US)
Pages (from-to)499-505
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume213
Issue number2
DOIs
StatePublished - Aug 15 1995
Externally publishedYes

Fingerprint

Cell membranes
Vitamin D
Cell Membrane
Pumps
Calcium
RNA
Transcription
Genes
Nuclear RNA
Calcitriol
Nuclear Envelope
Ribonucleases
Gene expression
Assays
Contamination
Hormones
Gene Expression
Experiments

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Vitamin D-Dependent Transcriptional Regulation of the Intestinal Plasma Membrane Calcium Pump. / Pannabecker, Thomas L; Chandler, J. S.; Wasserman, R. H.

In: Biochemical and Biophysical Research Communications, Vol. 213, No. 2, 15.08.1995, p. 499-505.

Research output: Contribution to journalArticle

@article{88567948cc014b7dac4b171311555ee1,
title = "Vitamin D-Dependent Transcriptional Regulation of the Intestinal Plasma Membrane Calcium Pump",
abstract = "The vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH) 2D3], was shown to increase intestinal plasma membrane calcium pump (PMCA) gene expression. The present study was done to determine whether gene transcription is involved in this process. Nuclei were isolated from duodena of vitamin-D-deficient chicks given 1,25(OH) 2D3 intracardially at various times before experiment. The abundance of PMCA RNA in the nuclear and total cellular fractions, measured by a ribonuclease protection assay, was significantly increased above control values at 1.5 hr. and maximized at 3 hr. post-dose. As shown, cross-contamination of nuclear PMCA RNA by cytosolic RNA cannot account for these results. These studies are the first to show that 1,25(OH) 2D3 regulates expression of a plasma membrane calcium pump gene by increasing the rate of transcription.",
author = "Pannabecker, {Thomas L} and Chandler, {J. S.} and Wasserman, {R. H.}",
year = "1995",
month = "8",
day = "15",
doi = "10.1006/bbrc.1995.2159",
language = "English (US)",
volume = "213",
pages = "499--505",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Vitamin D-Dependent Transcriptional Regulation of the Intestinal Plasma Membrane Calcium Pump

AU - Pannabecker, Thomas L

AU - Chandler, J. S.

AU - Wasserman, R. H.

PY - 1995/8/15

Y1 - 1995/8/15

N2 - The vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH) 2D3], was shown to increase intestinal plasma membrane calcium pump (PMCA) gene expression. The present study was done to determine whether gene transcription is involved in this process. Nuclei were isolated from duodena of vitamin-D-deficient chicks given 1,25(OH) 2D3 intracardially at various times before experiment. The abundance of PMCA RNA in the nuclear and total cellular fractions, measured by a ribonuclease protection assay, was significantly increased above control values at 1.5 hr. and maximized at 3 hr. post-dose. As shown, cross-contamination of nuclear PMCA RNA by cytosolic RNA cannot account for these results. These studies are the first to show that 1,25(OH) 2D3 regulates expression of a plasma membrane calcium pump gene by increasing the rate of transcription.

AB - The vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH) 2D3], was shown to increase intestinal plasma membrane calcium pump (PMCA) gene expression. The present study was done to determine whether gene transcription is involved in this process. Nuclei were isolated from duodena of vitamin-D-deficient chicks given 1,25(OH) 2D3 intracardially at various times before experiment. The abundance of PMCA RNA in the nuclear and total cellular fractions, measured by a ribonuclease protection assay, was significantly increased above control values at 1.5 hr. and maximized at 3 hr. post-dose. As shown, cross-contamination of nuclear PMCA RNA by cytosolic RNA cannot account for these results. These studies are the first to show that 1,25(OH) 2D3 regulates expression of a plasma membrane calcium pump gene by increasing the rate of transcription.

UR - http://www.scopus.com/inward/record.url?scp=0029142972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029142972&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.2159

DO - 10.1006/bbrc.1995.2159

M3 - Article

VL - 213

SP - 499

EP - 505

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -