War of the iguanas: Conflicting molecular and morphological phylogenies and long-branch attraction in iguanid lizards

John J Wiens, Bradford D. Hollingsworth

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Recent studies based on different types of data (i.e., morphology, molecules) have found strongly conflicting phylogenies for the genera of iguanid lizards but have been unable to explain the basis for this incongruence. We reanalyze published data from morphology and from the mitochondrial ND4, cytochrome b, 12S, and 16S genes to explore the sources of incongruence and resolve these conflicts. Much of the incongruence centers on the genus Cyclura, which is the sister taxon of Iguana, according to parsimony analyses of the morphology and the ribosomal genes, but is the sister taxon of all other Iguanini, according to the protein-coding genes. Maximum likelihood analyses show that there has been an increase in the rate of nucleotide substitution in Cyclura in the two protein-coding genes (ND4 and cytochrome b), although this increase is not as clear when parsimony is used to estimate branch lengths. Parametric simulations suggest that Cyclura may be misplaced by the protein-coding genes as a result of long-branch attraction; even when Cyclura and Iguana are sister taxa in a simulated phylogeny, Cyclura is still placed as the basal member of the Iguanini by parsimony analysis in 55% of the replicates. A similar long-branch attraction problem may also exist in the morphological data with regard to the placement of Sauromalus with the Galápagos iguanas (Amblyrhynchus and Conolophus). The results have many implications for the analysis of diverse data sets, the impact of long branches on parsimony and likelihood methods, and the use of certain protein-coding genes in phylogeny reconstruction.

Original languageEnglish (US)
Pages (from-to)143-159
Number of pages17
JournalSystematic Biology
Volume49
Issue number1
StatePublished - Mar 2000
Externally publishedYes

Fingerprint

Iguanas
Iguana (Iguanidae)
Lizards
Phylogeny
lizard
lizards
phylogeny
Cytochromes b
gene
Proteins
protein
genes
cytochrome b
cytochrome
proteins
Genes
parsimony analysis
Nucleotides
Warfare
data analysis

Keywords

  • Data set incongruence
  • Iguanidae
  • Likelihood
  • Long-branch attraction, parsimony

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

War of the iguanas : Conflicting molecular and morphological phylogenies and long-branch attraction in iguanid lizards. / Wiens, John J; Hollingsworth, Bradford D.

In: Systematic Biology, Vol. 49, No. 1, 03.2000, p. 143-159.

Research output: Contribution to journalArticle

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