Water-soluble nonionic surfactants for affinity bioseparations

Roberto Z Guzman, J. L. Torres, R. G. Carbonell, P. K. Kilpatrick

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Reversible competitive inhibitors of the three enzymes β-galactosidase, trypsin, and serum cholinesterase have been covalently attached to nonionic ethoxylated surfactants. The binding of the resulting affinity-derivatized surfactants to the respective enzymes has been quantified by measuring Michaelis-Menten inhibition constants with kinetic assays. The surfactant-inhibitor of serum cholinesterase, octaethylene glycol monohexadecyl ether pyridinium (C16E8-PYR), was adsorbed in aqueous solution to an octadecyl-bonded reverse-phase silica packing in a 2×0.2 cm stainless steel test column. The ability of the test column to function as a high-performance affinity chromatography (HPAC) column was determined by applying a mixture of bovine serum albumin and cholinesterase (4:1 w/w). Virtually all of the cholinesterase bound and was eluted by applying a gradient in ionic strength. The applied cholinesterase was covered with a yield of over 90% and an 11-fold purification.

Original languageEnglish (US)
Pages (from-to)1267-1276
Number of pages10
JournalBiotechnology and Bioengineering
Volume33
Issue number10
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Cholinesterases
Nonionic surfactants
Surface-Active Agents
Surface active agents
Enzymes
Enzyme inhibition
Affinity chromatography
Enzyme kinetics
Water
Glycols
Ionic strength
Galactosidases
Purification
Ethers
Assays
Stainless steel
Cholinesterase Inhibitors
Stainless Steel
Silica
Enzyme Inhibitors

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Guzman, R. Z., Torres, J. L., Carbonell, R. G., & Kilpatrick, P. K. (1989). Water-soluble nonionic surfactants for affinity bioseparations. Biotechnology and Bioengineering, 33(10), 1267-1276.

Water-soluble nonionic surfactants for affinity bioseparations. / Guzman, Roberto Z; Torres, J. L.; Carbonell, R. G.; Kilpatrick, P. K.

In: Biotechnology and Bioengineering, Vol. 33, No. 10, 01.01.1989, p. 1267-1276.

Research output: Contribution to journalArticle

Guzman, RZ, Torres, JL, Carbonell, RG & Kilpatrick, PK 1989, 'Water-soluble nonionic surfactants for affinity bioseparations', Biotechnology and Bioengineering, vol. 33, no. 10, pp. 1267-1276.
Guzman RZ, Torres JL, Carbonell RG, Kilpatrick PK. Water-soluble nonionic surfactants for affinity bioseparations. Biotechnology and Bioengineering. 1989 Jan 1;33(10):1267-1276.
Guzman, Roberto Z ; Torres, J. L. ; Carbonell, R. G. ; Kilpatrick, P. K. / Water-soluble nonionic surfactants for affinity bioseparations. In: Biotechnology and Bioengineering. 1989 ; Vol. 33, No. 10. pp. 1267-1276.
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